Localization Performance in a Binaural Real-Time Auralization System Extended to Research Hearing Aids

Auralization systems for auditory research should ideally be validated by perceptual experiments, as well as objective measures. This study employed perceptual tests to evaluate a recently proposed binaural real-time auralization system for hearing aid (HA) users. The dynamic localization of real sound sources was compared with that of virtualized ones, reproduced binaurally over headphones, loudspeakers with crosstalk cancellation (CTC) filters, research HAs, or combined via loudspeakers with CTC filters and research HAs under free-field conditions. System-inherent properties affecting localization cues were identified and their effects on overall horizontal localization, reversal rates, and angular error metrics were assessed. The general localization performance in combined reproduction was found to fall between what was measured for loudspeakers with CTC filters and research HAs alone. Reproduction via research HAs alone resulted in the highest reversal rates and angular errors. While combined reproduction helped decrease the reversal rates, no significant effect was observed on the angular error metrics. However, combined reproduction resulted in the same overall horizontal source localization performance as measured for real sound sources, while improving localization compared with reproduction over research HAs alone. Collectively, the results with respect to combined reproduction can be considered a performance indicator for future experiments involving HA users.

Subcellular localization of several structurally different tyrosine kinase inhibitors

Protein tyrosine kinases form an important target for a new class of anticancer drugs, the tyrosine kinase inhibitors (TKIs). Recently we demonstrated that sunitinib, an inhibitor of the membrane-associated vascular endothelial growth factor receptor (VEGFR), is trapped in lysosomes which isolates the drug from its intended target. Therefore we investigated whether this also holds for other TKIs, targeted against different protein kinases. For this purpose we used the ProteoExtractR kit, which enables a subcellular extraction separating cellular proteins into four distinct fractions covering the cytosol, membranes and membrane organelles (including lysosomes), nuclear proteins and the cytoskeleton. Since TKIs are 98-100 % protein bound we used this property to study their subcellular distribution and used Caco-2 cells as a model. As expected after 2 hours exposure sunitinib was trapped in cytosol (58 %) and organelles (42 % including lysosomes). Crizotinib, an inhibitor of ALK-EML4, showed a similar distribution. However, erlotinib, an inhibitor of the epidermal growth factor receptor (EGFR) showed a very low cellular accumulation and was limited to the organelle fraction. In contrast, the other EGFR inhibitor, gefitinib was predominantly located in the cytosolic (39 %) and membrane fraction (44 %). Sorafenib, another VEGFR inhibitor was predominantly located in the organelle fraction (85 %) and cytosol (15 %) after 2 hours, while after 24 hours distribution decreased (9.9 fold) with a slight shift. Dasatinib, an inhibitor of BCR-Abl was located only in the cytosol (100 %). In general localization after 24 hours was comparable, albeit several small changes were seen. In conclusion protein fractionation with the ProteoExtractR Subcellular Proteome Extraction kit demonstrated large differences in TKI levels in various cellular organelles, with a pattern in agreement with lysosomal accumulation of sunitinib.

The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar

The distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.

User Localization in Complex Environments by Multimodal Combination of GPS, WiFi, RFID, and Pedometer Technologies

Many user localization technologies and methods have been proposed for either indoor or outdoor environments. However, each technology has its own drawbacks. Recently, many researches and designs have been proposed to build a combination of multiple localization technologies system which can provide higher precision results and solve the limitation in each localization technology alone. In this paper, a conceptual design of a general localization platform using combination of multiple localization technologies is introduced. The combination is realized by dividing spaces into grid points. To demonstrate this platform, a system with GPS, RFID, WiFi, and pedometer technologies is established. Experiment results show that the accuracy and availability are improved in comparison with each technology individually.

Device Localization in Ubiquitous Computing Environments

In this chapter, we will study the localization problem in ubiquitous computing environments. In general, localization refers to the problem of obtaining (semi-) accurate physical location of the devices in a dynamic environment in which only a small subset of the devices know their exact location. Using localization techniques, other devices can indirectly derive their own location by means of some measurement data such as distance and angle to their neighbors. Localization is now regarded as an enabling technology for ubiquitous computing environments because it can substantially increase the performance of other fundamental tasks such as routing, energy conservation, and network security. Localization is also a difficult problem because it is computationally intractable. Furthermore, it has to be implemented in a highly dynamic and distributed environment in which measurement data is often subject to noise. In this chapter, we will give an overview of localization in terms of its common applications, its hardware capacities, its algorithms, and its computational complexity.

Device Localization in Ubiquitous Computing Environments

In this chapter, we will study the localization problem in ubiquitous computing environments. In general, localization refers to the problem of obtaining (semi-) accurate physical location of the devices in a dynamic environment in which only a small subset of the devices know their exact location. Using localization techniques, other devices can indirectly derive their own location by means of some measurement data such as distance and angle to their neighbors. Localization is now regarded as an enabling technology for ubiquitous computing environments because it can substantially increase the performance of other fundamental tasks such as routing, energy conservation, and network security. Localization is also a difficult problem because it is computationally intractable. Furthermore, it has to be implemented in a highly dynamic and distributed environment in which measurement data is often subject to noise. In this chapter, we will give an overview of localization in terms of its common applications, its hardware capacities, its algorithms, and its computational complexity.

An Upstream AG Determines Whether a Downstream AG Is Selected during Catalytic Step II of Splicing

ABSTRACT Specific mechanisms must exist to ensure fidelity in selecting the AG dinucleotide that functions as the 3′ splice site during the second transesterification step of splicing. Here we show that the optimal location for this AG is within a narrow distance (19 to 23 nucleotides [nt]) downstream from the branch point sequence (BPS). Contrary to previous expectations, AGs located less than 23 nt from the BPS are always recognized, even when a second AG located more optimally downstream is used in the transesterification reaction. Indeed, the AG closest to the BPS actually dictates the precise location of the AG that engages in the reaction. This mechanism, in which the AG is identified by a general localization step followed by a precise localization step, may be used to achieve fidelity while allowing flexibility in the location of 3′ splice sites.