Experimental study on the mechanical properties of biological hydrogels of different concentrations

BACKGROUND: Biological hydrogels provide a conducive three-dimensional extracellular matrix environment for encapsulating and cultivating living cells. Microenvironmental modulus of hydrogels dictates several characteristics of cell functions such as proliferation, adhesion, self-renewal, differentiation, migration, cell morphology and fate. Precise measurement of the mechanical properties of gels is necessary for investigating cellular mechanobiology in a variety of applications in tissue engineering. Elastic properties of gels are strongly influenced by the amount of crosslinking density. OBJECTIVE: The main purpose of the present study was to determine the elastic modulus of two types of well-known biological hydrogels: Agarose and Gelatin Methacryloyl. METHODS: Mechanical properties such as Young’s modulus, fracture stress and failure strain of the prescribed gels with a wide range of concentrations were determined using tension and compression tests. RESULTS: The elastic modulus, failure stress and strain were found to be strongly influenced when the amount of concentration in the hydrogels was changed. The elastic modulus for a lower level of concentration, not considered in this study, was also predicted using statistical analysis. CONCLUSIONS: Closed matching of the mechanical properties of the gels revealed that the bulk tension and compression tests could be confidently used for assessing mechanical properties of delicate biological hydrogels.

Cell Nanomechanics Based on Dielectric Elastomer Actuator Device

As a frontier of biology, mechanobiology plays an important role in tissue and biomedical engineering. It is a common sense that mechanical cues under extracellular microenvironment affect a lot in regulating the behaviors of cells such as proliferation and gene expression, etc. In such an interdisciplinary field, engineering methods like the pneumatic and motor-driven devices have been employed for years. Nevertheless, such techniques usually rely on complex structures, which cost much but not so easy to control. Dielectric elastomer actuators (DEAs) are well known as a kind of soft actuation technology, and their research prospect in biomechanical field is gradually concerned due to their properties just like large deformation (> 100%) and fast response (< 1 ms). In addition, DEAs are usually optically transparent and can be fabricated into small volume, which make them easy to cooperate with regular microscope to realize real-time dynamic imaging of cells. This paper first reviews the basic components, principle, and evaluation of DEAs and then overview some corresponding applications of DEAs for cellular mechanobiology research. We also provide a comparison between DEA-based bioreactors and current custom-built devices and share some opinions about their potential applications in the future according to widely reported results via other methods.