eastern fence lizard
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2020 ◽  
Author(s):  
Aundrea K. Westfall ◽  
Rory S. Telemeco ◽  
Mariana B. Grizante ◽  
Damien S. Waits ◽  
Amanda D. Clark ◽  
...  

AbstractHigh-quality genomic resources facilitate population-level and species-level comparisons to answer questions about behavioral ecology, morphological and physiological adaptations, as well as the evolution of genomic architecture. Squamate reptiles (lizards and snakes) are particularly diverse in characteristics that have intrigued evolutionary biologists, but high-quality genomic resources for squamates are relatively sparse. Lizards in the genus Sceloporus have a long history as important ecological, evolutionary, and physiological models, making them a valuable target for the development of genomic resources. We present a high-quality chromosome-level reference genome assembly, SceUnd1.0, (utilizing 10X Genomics Chromium, HiC, and PacBio data) and tissue/developmental stage transcriptomes for the Eastern Fence Lizard, Sceloporus undulatus. We performed synteny analysis with other available squamate chromosome-level assemblies to identify broad patterns of chromosome evolution including the fusion of micro- and macrochromosomes in S. undulatus. Using this new S. undulatus genome assembly we conducted reference-based assemblies for 34 other Sceloporus species to improve draft nuclear genomes assemblies from 1% coverage to 43% coverage on average. Across these species, typically >90% of reads mapped for species within 20 million years divergence from S. undulatus, this dropped to 75% reads mapped for species at 35 million years divergence. Finally we use RNAseq and whole genome resequencing data to compare the three assemblies as references, each representing an increased level of sequencing, cost and assembly efforts: Supernova Assembly with data from10X Genomics Chromium library; HiRise Assembly that added data from HiC library; and PBJelly Assembly that added data from PacBio sequencing. We found that the Supernova Assembly contained the full genome and was a suitable reference for RNAseq, but the chromosome-level scaffolds provided by the addition of the HiC data allowed the reference to be used for other whole genome analysis, including synteny and whole genome association mapping analyses. The addition of PacBio data provided negligible gains. Overall, these new genomic resources provide valuable tools for advanced molecular analysis of an organism that has become a model in physiology and evolutionary ecology.



2018 ◽  
Vol 279 (11) ◽  
pp. 1629-1639 ◽  
Author(s):  
Daniel G. Blackburn ◽  
Luisa Lestz ◽  
Madeline S. Barnes ◽  
Kathryn G. Powers ◽  
Tracy Langkilde


Ethology ◽  
2018 ◽  
Vol 124 (9) ◽  
pp. 684-690 ◽  
Author(s):  
Alison G. Ossip-Drahos ◽  
Nicolas J. Berry ◽  
Christian M. King ◽  
Emília P. Martins


2017 ◽  
Author(s):  
Mariana B. Grizante ◽  
Marc Tollis ◽  
Juan J. Rodriguez ◽  
Ofir Levy ◽  
Michael J. Angilletta ◽  
...  

AbstractBackgroundThe eastern fence lizard (Sceloporus undulatus) has been a model species for ecological and evolutionary research. Genomic and transcriptomic resources for this species would promote investigation of genetic mechanisms that underpin plastic responses to environmental stress, such as climate warming. Moreover, such resources would aid comparative studies of complex traits at the molecular level, such as the transition from oviparous to viviparous reproduction, which happened at least four times within Sceloporus.FindingsA de novo transcriptome assembly for Sceloporus undulatus, Sund_v1.0, was generated using over 179 million Illumina reads obtained from three tissues (whole brain, skeletal muscle, and embryo) as well as previously reported liver sequences. The Sund_v1.0 assembly had an average contig length of 782 nucleotides and an E90N50 statistic of 2,550 nucleotides. Comparing S. undulatus transcripts with the benchmarking universal single-copy orthologs (BUSCO) for tetrapod species yielded 97.2% gene representation. A total of 13,422 protein-coding orthologs were identified in comparison to the genome of the green anole lizard, Anolis carolinensis, which is the closest related species with genomic data available.ConclusionsThe multi-tissue transcriptome of S. undulatus is the first for a member of the family Phrynosomatidae, offering an important resource to advance studies of adaptation in this species and genomic research in reptiles.



2016 ◽  
Vol 50 (2) ◽  
pp. 284-288 ◽  
Author(s):  
Christopher J. Thawley ◽  
Tracy Langkilde


Micron ◽  
2016 ◽  
Vol 81 ◽  
pp. 16-22 ◽  
Author(s):  
Justin L. Rheubert ◽  
David M. Sever ◽  
Dustin S. Siegel ◽  
Kevin M. Gribbins


2014 ◽  
Vol 9 (3) ◽  
pp. 360-371 ◽  
Author(s):  
Weiguo DU ◽  
Travis R. ROBBINS ◽  
Daniel A. WARNER ◽  
Tracy LANGKILDE ◽  
Richard SHINE




2012 ◽  
Vol 46 (2) ◽  
pp. 162-166 ◽  
Author(s):  
R. L. Rosier ◽  
T Langkilde


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