fluorescence nanoscopy
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Author(s):  
Luciano A. Masullo ◽  
Alan M. Szalai ◽  
Lucía F. Lopez ◽  
Fernando D. Stefani

Engineering ◽  
2021 ◽  
Author(s):  
Yangyundou Wang ◽  
Jian Lin ◽  
Qiming Zhang ◽  
Xi Chen ◽  
Haitao Luan ◽  
...  

Engineering ◽  
2020 ◽  
Author(s):  
Xiang Hao ◽  
Yiming Li ◽  
Shuang Fu ◽  
Yanghui Li ◽  
Yingke Xu ◽  
...  

2020 ◽  
Vol 124 (35) ◽  
pp. 7525-7536
Author(s):  
Taku Furubayashi ◽  
Keita Ishida ◽  
Eiji Nakata ◽  
Takashi Morii ◽  
Kanta Naruse ◽  
...  

Author(s):  
Flavie Lavoie-Cardinal ◽  
Anthony Bilodeau ◽  
Mado Lemieux ◽  
Marc-André Gardner ◽  
Theresa Wiesner ◽  
...  

AbstractThe nanoscale organization of the F-actin cytoskeleton in neurons comprises membrane-associated periodical rings, bundles, and longitudinal fibers. The F-actin rings have been observed predominantly in axons but only sporadically in dendrites, where fluorescence nanoscopy reveals various patterns of F-actin arranged in mixed patches. These complex dendritic F-actin patterns pose a challenge for investigating quantitatively their regulatory mechanisms. We developed here a weakly supervised deep learning segmentation approach of fluorescence nanoscopy images of F-actin in cultured hippocampal neurons. This approach enabled the quantitative assessment of F-actin remodeling, revealing the disappearance of the rings during neuronal activity in dendrites, but not in axons. The dendritic F-actin cytoskeleton of activated neurons remodeled into longitudinal fibers. We show that this activity-dependent remodeling involves Ca2+ and NMDA-dependent mechanisms. This highly dynamic restructuring of dendritic F-actin based submembrane lattice into longitudinal fibers may serve to support activity-dependent membrane remodeling, protein trafficking and neuronal plasticity.


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