The costs of being a good dad: egg-carrying and clutch size impair locomotor performance in male midwife toads (Alytes obstetricans)

Parental care is widespread across the animal kingdom. Parental behaviours are beneficial by increasing offspring survival but induce significant costs to the parents. Because parental care is far more common in females, the associated reproductive costs have been largely studied in this sex. Although male parental care is likely to involve significant costs, it has been markedly less well investigated. We studied the costs of egg-carrying on locomotor performance in an amphibian species (Alytes obstetricans) with male parental care. We examined complementary parameters including hopping performance, righting response, hindleg muscle response to egg burden, and homing time in males carrying or not carrying eggs. We found that carrying males showed altered locomotor performance for most traits. In addition, alteration of performance was closely related to relative clutch size. Clutch desertion occurred in smaller individuals carrying larger relative clutch mass, and performance after desertion was similar to that of non-reproductive individuals. Overall, our study demonstrates that carrying eggs significantly alters male mobility and that performance–clutch size trade-offs are relevant in understanding the evolution of paternal care.

Hybrid zone genomics supports candidate species in Iberian Alytes obstetricans

While estimates of genetic divergence are increasingly used in molecular taxonomy, hybrid zone analyses can provide decisive evidence for evaluating candidate species. Applying a population genomic approach (RAD-sequencing) to a fine-scale transect sampling, we analyzed the transition between two Iberian subspecies of the common midwife toad (Alytes obstetricans almogavarii and A. o. pertinax) in Catalonia (northeastern Spain), which putatively diverged since the Plio-Pleistocene. Their hybrid zone was remarkably narrow, with extensive admixture restricted to a single locality (close to Tarragona), and congruent allele frequency clines for the mitochondrial (13 km wide) and the average nuclear genomes (16 km wide). We also fitted clines independently for 89 taxon-diagnostic SNPs: most of them behave like the nuclear background, but a subset (13%) is completely impermeable to gene flow and might be linked to barrier loci involved in hybrid incompatibilities. Assuming that midwife toads are able to disperse in the area of contact, we conclude that these taxa experience partial reproductive isolation and represent incipient species, i.e. Alytes almogavarii and Alytes obstetricans. Interestingly, their evolutionary age and mitochondrial divergence fall below the thresholds proposed in molecular systematics studies, emphasizing the difficulty of predicting the outcome of secondary contacts between young lineages entering the grey zone of speciation.

Midwife toads (Alytes obstetricans) select their diurnal refuges based on hydric and thermal properties

Most amphibians use both aquatic and terrestrial habitats. While the aquatic phase attracted considerable interest, terrestrial habitat use is often less investigated. We studied diurnal refuge selection in the Midwife toad in Western central France. We used a factorial design and tested the effect of refuge type (wood versus rubber boards) and substrate (wet sawdust versus bare soil). Most animals were observed under refuges with sawdust substrate. An interaction between refuge type and temperature was detected with higher presence probability under rubber refuges at low temperature. Conflicting hydric and thermic requirements are likely determinants of the observed pattern.

Background colour matching in a wild population of Alytes obstetricans

The capacity for physiological colour change has long been described in anuran amphibians. Camouflage against predators seems to be the most relevant function of dynamic changes in skin colour of frogs, but key aspects such as the rate at which these changes occur, or the specific colour components involved are not completely clear. Whereas most research on the topic has been reported on tree frogs in laboratory conditions, studies in other anurans or in the field are much scarcer. Here we show a potentially plastic, adaptive response in coloration of common midwife toads, Alytes obstetricans, from a population of central Portugal, whose pigmentation varied with their natural backgrounds. Using quantitative image analysis, we compared hue, saturation and brightness of dorsal skin coloration of toads and the colour of the area of ground immediately around them. We found a positive correlation between coloration of toads and background colour for the three components of the colour. As well as other anuran species, A. obstetricans might adjust skin coloration to match the surrounding environment, thus benefitting from short-term reversible crypsis strategies against predators. A less supported hypothesis would be that toads accurately select matching backgrounds to improve concealment as an antipredatory strategy.