Broadband 75–85 MHz radiofrequency fields disrupt magnetic compass orientation in night-migratory songbirds consistent with a flavin-based radical pair magnetoreceptor

The light-dependent magnetic compass sense of night-migratory songbirds can be disrupted by weak radiofrequency fields. This finding supports a quantum mechanical, radical-pair-based mechanism of magnetoreception as observed for isolated cryptochrome 4, a protein found in birds’ retinas. The exact identity of the magnetically sensitive radicals in cryptochrome is uncertain in vivo, but their formation seems to require a bound flavin adenine dinucleotide chromophore and a chain of four tryptophan residues within the protein. Resulting from the hyperfine interactions of nuclear spins with the unpaired electrons, the sensitivity of the radicals to radiofrequency magnetic fields depends strongly on the number of magnetic nuclei (hydrogen and nitrogen atoms) they contain. Quantum-chemical calculations suggested that electromagnetic noise in the frequency range 75–85 MHz could give information about the identity of the radicals involved. Here, we show that broadband 75–85 MHz radiofrequency fields prevent a night-migratory songbird from using its magnetic compass in behavioural experiments. These results indicate that at least one of the components of the radical pair involved in the sensory process of avian magnetoreception must contain a substantial number of strong hyperfine interactions as would be the case if a flavin–tryptophan radical pair were the magnetic sensor.

Five-State Extended Kalman Filter for Estimation of Speed over Ground (SOG), Course over Ground (COG) and Course Rate of Unmanned Surface Vehicles (USVs): Experimental Results

Small USVs are usually equipped with a low-cost navigation sensor suite consisting of a global navigation satellite system (GNSS) receiver and a magnetic compass. Unfortunately, the magnetic compass is highly susceptible to electromagnetic disturbances. Hence, it should not be used in safety-critical autopilot systems. A gyrocompass, however, is highly reliable, but it is too expensive for most USV systems. It is tempting to compute the heading angle by using two GNSS antennas on the same receiver. Unfortunately, for small USV systems, the distance between the antennas is very small, requiring that an RTK GNSS receiver is used. The drawback of the RTK solution is that it suffers from dropouts due to ionospheric disturbances, multipath, interference, etc. For safety-critical applications, a more robust approach is to estimate the course angle to avoid using the heading angle during path following. The main result of this article is a five-state extended Kalman filter (EKF) aided by GNSS latitude-longitude measurements for estimation of the course over ground (COG), speed over ground (SOG), and course rate. These are the primary signals needed to implement a course autopilot system onboard a USV. The proposed algorithm is computationally efficient and easy to implement since only four EKF covariance parameters must be specified. The parameters need to be calibrated for different GNSS receivers and vehicle types, but they are not sensitive to the working conditions. Another advantage of the EKF is that the autopilot does not need to use the COG and SOG measurements from the GNSS receiver, which have varying quality and reliability. It is also straightforward to add complementary sensors such as a Doppler Velocity Log (DVL) to the EKF to improve the performance further. Finally, the performance of the five-state EKF is demonstrated by experimental testing of two commercial USV systems.

UNIVERSAL MEASURING MODULE BASED ON A THREE-COMPONENT FERROSONDE SENSOR AND A MAGNETIZATION STATION BASED ON ITS BASIS

The work is devoted to the description of the design of the measuring module (fluxgate magnetic compass) and the magnetovariation station (geophysical device) created on its basis. This station is designed to carry out geomagnetic work in various conditions - both stationary observation points and for work in the field.

A magnet attached to the forehead disrupts magnetic compass orientation in a migratory songbird

For studies on magnetic compass orientation and navigation performance in small bird species, controlled experiments with orientation cages inside an electromagnetic coil system are the most prominent methodological paradigm. These are, however, not applicable when studying larger bird species and/or orientation behaviour during free flight. For this, researchers have followed a very different approach. By attaching small magnets to birds, they intended to deprive them of access to meaningful magnetic information. Unfortunately, results from studies using this approach appear rather inconsistent. As these are based on experiments with birds under free flight conditions, which usually do not allow exclusion of other potential orientation cues, an assessment of the overall efficacy of this approach is difficult to conduct. Here, we directly test the efficacy of small magnets for temporarily disrupting magnetic compass orientation in small migratory songbirds using orientation cages under controlled experimental conditions. We found that birds which have access to the Earth's magnetic field as their sole orientation cue show a general orientation towards their seasonally appropriate migratory direction. When carrying magnets on their forehead under these conditions, the same birds become disoriented. However, under changed conditions that allow birds access to other (i.e. celestial) orientation cues, any disruptive effect of the magnets they carry appears obscured. Our results provide clear evidence for the efficacy of the magnet approach for temporarily disrupting magnetic compass orientation in birds, but also reveal its limitations for application in experiments under free flight conditions.

Orientations and water dynamics of photoinduced secondary charge-separated states for magnetoreception by cryptochrome

In the biological magnetic compass, blue-light photoreceptor protein of cryptochrome is thought to conduct the sensing of the Earth’s magnetic field by photoinduced sequential long-range charge-separation (CS) through a cascade of tryptophan residues, WA(H), WB(H) and WC(H). Mechanism of generating the weak-field sensitive radical pair (RP) is poorly understood because geometries, electronic couplings and their modulations by molecular motion have not been investigated in the secondary CS states generated prior to the terminal RP states. In this study, water dynamics control of the electronic coupling is revealed to be a key concept for sensing the direction of weak magnetic field. Geometry and exchange coupling (singlet–triplet energy gap: 2J) of photoinduced secondary CS states composed of flavin adenine dinucleotide radical anion (FAD−•) and radical cation WB(H)+• in the cryptochrome DASH from Xenopus laevis were clarified by time-resolved electron paramagnetic resonance. We found a time-dependent energetic disorder in 2J and was interpreted by a trap CS state capturing one reorientated water molecule at 120 K. Enhanced electron-tunneling by water-libration was revealed for the terminal charge-separation event at elevated temperature. This highlights importance of optimizing the electronic coupling for regulation of the anisotropic RP yield on the possible magnetic compass senses.

The retinal circuitry for magnetoreception in migratory birds

Night-migratory birds use the Earth’s magnetic field to determine the direction in which they want to migrate. Many studies suggest that this “magnetic compass sense” is light dependent and mediated by blue light sensors, called cryptochromes, which are expressed in the retina of night-migratory birds. In this review, we summarize the evidence that the avian retina processes not only visual information but also magnetic compass information. We also review the current knowledge on cryptochrome expression in the bird retina and highlight open questions which we aim to address within the framework of SFB 1372 Magnetoreception and Navigation in Vertebrates.

Cryptochrome expression in avian UV cones: revisiting the role of CRY1 as magnetoreceptor

Cryptochromes (CRY) have been proposed as putative magnetoreceptors in vertebrates. Localisation of CRY1 in the UV cones in the retinas of birds suggested that it could be the candidate magnetoreceptor. However, recent findings argue against this possibility. CRY1 is a type II cryptochrome, a subtype of cryptochromes that may not be inherently photosensitive, and it exhibits a clear circadian expression in the retinas of birds. Here, we reassessed the localisation and distribution of CRY1 in the retina of the zebra finch. Zebra finches have a light-dependent magnetic compass based on a radical-pair mechanism, similar to migratory birds. We found that CRY1 colocalised with the UV/V opsin (SWS1) in the outer segments of UV cones, but restricted to the tip of the segments. CRY1 was found in all UV cones across the entire retina, with the highest densities near the fovea. Pre-exposure of birds to different wavelengths of light did not result in any difference in CRY1 detection, suggesting that CRY1 did not undergo any detectable functional changes as result of light activation. Considering that CRY1 is likely not involved in magnetoreception, our findings open the possibility for an involvement in different, yet undetermined functions in the avian UV/V cones.