Institutions devote significant resources to gender diversity and inclusion, yet little empirical research has examined the benefits of male allyship in higher education. Using women’s other- and self-reports and men’s self-reports from 101 male-female colleague dyads in male-dominated departments, we tested a model involving men’s allyship, women’s inclusion and vitality, and men’s growth and work-family enrichment. As hypothesized, men’s growth mediated the link between their allyship and work-family enrichment, and women’s perceptions of men’s allyship predicted women’s vitality, both directly and through inclusion. However, men’s allyship was weakly associated with women’s perceptions of their allyship, and men’s benefits were unrelated to women’s inclusion or vitality. Findings highlight the importance of male allyship while underscoring disconnect between women’s and men’s experiences.