The Consequences of Watching

The discussion of surveillance invariably leads to an assessment of the idea of privacy. This is a construct that has been widely debated over time, with some of the early aspects of privacy taking shape in Britain. Later, a Supreme Court judge in America argued for privacy as the right to be “left alone.” Over time, the concept has seen transformations with the focus on defining what aspects of the narrative can be considered to be protected. Eventually, every context of surveillance such as I2P and P2I has slightly different considerations of privacy.

Endosomal and lysosomal immunoprecipitation for proteomics, lipidomics, and TEM v1

Previous studies have developed methods for isolation of lysosomes, mitochondria, and peroxisomes from non-denaturing extracts. Here we describe an approach for purification of early/sorting endosomes, providing a means by which to examine early aspects of the endolysosomal system and to combine this with lysosome purification using Lyso-IP. We refer to this method as Endo-IP. This allows us to examine the proteome, lipidome, as well as electron microscopy imaging of endosomes.

Early Gross Motor Milestones in Duchenne Muscular Dystrophy

Background: Over the last few years there has been increasing attention to detect early signs of impairment in young Duchenne muscular dystrophy boys but less has been reported on whether the delay may also affect the very early aspects of motor development, such as gross motor milestones. Objective: The aim of this study was to retrospectively assess the age when early motor milestones were achieved in Duchenne muscular dystrophy. Methods: The study is a retrospective analysis of data collected as part of a larger natural history project. Information on past medical history, collected at the time the boys were seen for the first time, were recorded and re available on clinical notes and on electronic CRF. Results: Data were collected in 134 DMD boys. Sitting was achieved at 7.04 months. The % of DMD boys not achieving sitting by 9.4 months was 10%, ranging from 2% in the boys with mutations before exon 44 to 33% in those beyond exon 63. Walking was achieved at a mean age of 16.35 months. The % of DMD boys not achieving independent walking by 18 months was 17%, ranging from 9% in the boys with mutations between 44 and 51 to 42% in those beyond exon 63. Conclusions: Our results showed that the risk of a delay in sitting and walking was increasingly high in patients with mutations predictive of the involvement of different brain dystrophin isoforms.

Jack London, John Barleycorn (1913): truth

This chapter places Jack London’s autobiography John Barleycorn: Alcoholic Memoirs as the key text for understanding the figure of the Existential drinker. It is one of the first all-out formulations of the writer-as-drinker, mixing the nineteenth-century temperance view of the habitual drinker who is a moral failure with the image of himself as a drinker who can attain truths not available to the fall-in-the-gutter drunkard, nor indeed available to the run-of-the-mill sober citizen. The chapter deals with London’s idea of ‘the white logic’, that is, the attraction of alcohol as a means to enlightenment, while at the same time acknowledging that to choose this path is also to choose death. The chapter therefore covers questions of mortality, finitude, types of drinkers and drunkenness, early aspects of Existential philosophy (London partly draws on Nietzsche), as well as beginning consideration of the writer in relation to texts where drinking is central.

Soporte para la ingeniería de requerimientos orientada a aspectos en documentos de gran tamaño

La ingeniería de requerimientos es una tarea fundamental en el proceso de comprensión de cómo debe comportarse el sistema que está siendo construido. Los sistemas complejos incluyen cientos o miles de requerimientos sobre múltiples incumbencias, tanto funcionales como no funcionales. Para algunos sistemas, las fuentes de requerimientos son muchas, resultando en varios -en muchos casos extensos- documentos. En ciertos casos, los requerimientos coinciden de forma directa con las incumbencias de la aplicación, lo que les permite ser limpiamente encapsulados en diferentes módulos del sistema resultante. Sin embargo es común encontrar situaciones donde esta premisa no se cumple, tomando por ejemplo la obligación de registrar (logging) todas las acciones del sistema. La correspondiente incumbencia, el registro, no puede ser limpiamente encapsulada en un módulo, ya que afecta a muchas de las partes del sistema. Las incumbencias transversales atraviesan la estructura de requerimientos a lo largo de los diferentes documentos que componen la especificación del sistema. El seguimiento de este tipo de incumbencias a lo largo de todo el documento de requerimientos, viendo a qué requerimiento afecta, es una tarea compleja. AORE ataca la problemática de los requerimientos complejos cuando estos son difíciles o imposibles de encapsular en módulos separados. Conocida también esta práctica como Early Aspects, AORE modela una primera impresión de estas incumbencias transversales como aspectos (en sentido de Aspect Oriented Programming), identificando y caracterizando la influencia que cada uno tiene sobre otros requerimientos del sistema. Estos requerimientos que afectan a muchos otros se los conocen con el nombre de Cross Cutting Concerns (CCC). El trabajo desarrolla una herramienta para el seguimiento de las incumbencias transversales, para ayudar al Ingeniero de Requerimientos.