Genetic Basis for Lentil Adaptation to Summer Cropping in Northern Temperate Environments

ABSTRACTThe continued success of lentil (Lens culinaris Medik.) genetic improvement relies on the availability of broad genetic diversity and new alleles need to be identified and incorporated into the cultivated gene pool. Availability of robust and predictive markers greatly enhances the precise transfer of genomic regions from unadapted germplasm. Quantitative trait loci (QTLs) for key phenological traits in lentil were located using a recombinant inbreed line (RIL) population derived from a cross between an Ethiopian landrace (ILL 1704) and a northern temperate cultivar (CDC Robin). Field experiments were conducted at Sutherland research farm in Saskatoon and at Rosthern, Saskatchewan, Canada during 2018 and 2019. A linkage map was constructed using 21,634 SNPs located on seven linkage groups (LGs) which correspond to the seven haploid chromosomes of lentil. Eight QTL were identified for six phenological traits. Flowering related QTL were identified at two regions on LG6. FLOWERING LOCUS T (FT) genes were annotated within the flowering time QTL interval based on the lentil reference genome. Similarly, a major QTL for post-flowering developmental processes was located on LG5 with several senescence-associated genes annotated within the QTL interval. The flowering time QTL was validated in a different genetic background indicating the potential use of the identified markers for marker-assisted selection to precisely transfer genomic regions from exotic germplasm into elite crop cultivars without disrupting adaptation.Core IdeasStable QTL were located for key phenological traits in lentil that lead to regional adaptation.FT genes are candidates for controlling flowering time in lentil grown in temperate environments.A major locus controlling post-flowering developmental processes was located on lentil LG5 with several senescence-associated genes annotated within the QTL interval.Markers identified in this study can be useful for marker-assisted selection to precisely transfer genomic regions from exotic germplasm into elite lentil cultivars without disrupting adaptation.

Seleksi dan Konfirmasi Alel Gen-gen Hd pada Padi Berumur Genjah dan Produktivitas Tinggi Persilangan Code x Nipponbare

<p>To support the IP 300 program rice varieties<br />with both early maturity and high productivity are needed.<br />The objective of the research was to improve those traits in<br />Code variety using RIL (recombinant inbreed line) methods.<br />The research was conducted in the year 2009-2011 at the<br />greenhouse and laboratory of Molecular Biology, Indonesian<br />Center for Agricultural Biotechnology and Genetic<br />Resources Research and Development. Materials consisted<br />of 600 individuals of F2 derived from one of F1 Code x<br />Nipponbare crossed plants and they were planted up to F4<br />with seed-to-seed, then confirmed by 8 microsatelite<br />markers linked to loci of Hd genes. The molecular analysis<br />showed that 84 of 600 F2 plants produced. The F4 plants have<br />50% of flowering time shorter than F2 and F3 plants. Two<br />lines (CdNb_388 and CdNb_270) have higher productivity<br />compared to Code by the number of productive tillers (20)<br />and the number of tiller grains (2240 and 1740) and have<br />closely 50% of 60 days flowering time of Nipponbare. Three<br />lines of F4 plants (CdNb_270, 364, and 388) were predicted<br />to have allele of Hd7 gene, and CdNb_472 was predicted to<br />have alelle of Hd14 gene.</p>