Prioritizing Sex Recognition Over Learned Species Recognition: Hierarchical Mate Recognition in an Invasive Fish

Mate recognition is the process of identifying and assessing the appropriate species, sex or population of another individual for their suitability as a potential mate. Recognition may be innate or learned. Learning, the acquisition of knowledge or skills through experience, involves a relatively long-term change in behavioral responses. In this study we examined learned and innate mate recognition in invasive male mosquitofish, Gambusia holbrooki, interacting with female conspecifics and male and female native toothcarp, Aphanius iberus. Male mosquitofish directed no mating attempts at male toothcarp whereas numerous attempts were made toward female toothcarp. Male mosquitofish therefore differentiated between males and females, but initially did not distinguish between con- and heterospecific females. Neither the presence of a male toothcarp, nor the presence of a refugia affected the number of mating attempts received by females. However, by the second day males appeared to learn to more accurately direct their mating attempts, with larger female mosquitofish receiving the most attention, though smaller toothcarp females were still harassed. We propose that male mosquitofish, with a coercive mating system, are selected for persistence despite rejection by potential mates. In this scenario, the pool of potential mates may include heterospecifics whose avoidance of mating attempts may be ignored by male mosquitofish. It may thus be adaptive for male mosquitofish to prioritize sex recognition over species recognition: if one sex is recognized as a “non-mate” this will cut 50% from the pool of potential mates whereas recognition of a single species will remove many fewer potential mates from the pool. This innate sex recognition together with rapid learning of species identity may be a factor in the invasive success of mosquitofish.

Male coercion and female injury in a sexually cannibalistic mantis

Sexual conflict can generate coercive traits in males that enhance mating success at the expense of female fitness. Pre-copulatory sexual cannibalism—where females consume males without mating—typically favours cautious rather than coercive mating tactics, and few examples of the latter are known. Here, we show that males of the highly cannibalistic springbok mantis, Miomantis caffra , wrestle females during pre-mating interactions. We find that most initial contacts between males and females involve a violent struggle whereby each sex tries be the first to grasp hold of the other with their raptorial forelegs. When females win the struggle, they always cannibalize males. However, when males grasp females first, they dramatically increase the chance of mating. We also find striking evidence that, on some occasions, males wound females with their fore-tibial claws during struggles, resulting in haemolymph loss and scar tissue formation. Taken together, our results show how males can overcome the threat of cannibalism by coercively wrestling females. We argue that pre-copulatory injury in this species is likely to be a negative pleiotropic side-effect of coercive mating behaviour and foraging morphology.

Nuptial feeding and genital coupling of Neopanorpa scorpionflies (Insecta: Mecoptera: Panorpidae) with notal organs of various lengths

Neopanorpa, the second largest genus in Panorpidae, is mainly characterized by the well-developed notal organ on male tergum III. However, it remains largely unknown how the length of the notal organ influences the nuptial feeding behaviour of Neopanorpa. Here, we investigated the nuptial feeding by comparing the morphology of mating-related structures and the genital coupling of a) Neopanorpa lui Chou & Ran, 1981 with a weakly-developed notal organ, b) N. carpenteri Cheng, 1957 with a medium-sized notal organ, and c) N. longiprocessa Hua & Chou, 1997 with an extremely elongated notal organ. The couples of N. lui and N. carpenteri maintain an intermittent mouth-to-mouth mode but do not exchange any edible food. After that the males secrete a salivary mass onto the surface as a nuptial gift, which is distinctly larger in N. carpenteri than in N. lui. Correspondingly, the male salivary glands are more developed in N. carpenteri than in N. lui. Males of N. longiprocessa bear very short salivary glands corresponding to a coercive mating tactic. The genital couplings are similar among the three species of Neopanorpa. The paired hypovalves of males are used to control the cerci of females. The prominent basal processes of male gonostyli grasp the posterior portion of the female medigynium across the intersegmental membrane. The male aedeagus physically couples with the female medigynium to ensure the male phallotreme to connect to the female copulatory pore. The influence of the notal organ length on the nuptial feeding behaviour of Neopanorpa is briefly discussed.

Mating and aggressive behaviour of Brachyrhaphis olomina (Cyprinodontiformes: Poeciliidae)

Despite the increasing interest in the use of intromittent male genitalia and coercive mating behaviour in poeciliids, detailed studies of the mating behaviour of most species in this family are lacking. We describe here the mating and aggressive behaviours of Brachyrhaphis olomina, and correlate them with the condition of the female’s ovum and embryos (immature, mature and pregnant). B. olomina performed a wide range of aggressive (sidle spread, tail beating, coordinate) and mating behaviours (approximation, touch, lateral display, touch-lateral display). Some behaviours (e.g. tail beating) are shared with other poeciliids, but two sexual behaviours (touch and lateral display) and one aggressive (coordinate) behaviour may be unique to B. olomina and were not reported in a previous study. Differences in male behaviour when paired with a female with mature ovum (more mating displays, no agonistic movements) suggest that males detect the female’s reproductive condition from some distance. The distinctive nature of mating behaviour in B. olomina highlights the importance of studying different species to have a better understanding of the evolution of mating and aggressive behaviours in poeciliids. Digital video images related to the article are available at http://www.momo-p.com/showdetail-e.php?movieid=momo170720bo01a, http://www.momo-p.com/showdetail-e.php?movieid=momo170720bo02a and http://www.momo-p.com/showdetail-e.php?movieid=momo170720bo03a.

Patterns of intraspecific aggression inferred from injuries in an aquatic turtle with male-biased size dimorphism

Patterns of sexual size dimorphism (SSD) in turtles are correlated with ecological mode, and it has been hypothesized that mating systems are also shaped by ecological mode. Male combat and coercive mating are competing explanations for male-biased SSD, but are difficult to assess empirically in aquatic species with cryptic behaviour. We quantified SSD and compiled observations of putative combat wounds collected from over 500 captures of Snapping Turtles (Chelydra serpentina (L., 1758)) in Algonquin Provincial Park, Ontario, to test hypotheses of mate competition and coercion. We found that both sex and body size were important predictors of risk of wounding, consistent with the hypothesis that male–male sexual competition is the primary driver of intraspecific aggression. Low wounding rates among females suggests that resource competition and coercive mating are not important causes of injuries. The risk of wounding increased monotonically with body size in adult males but not in adult females, and small males were less likely to be injured, suggesting that they employ a risk-averse strategy by avoiding direct competition for mates. There was no evidence of asymptotic or decreasing wounding probability in the largest males, which is consistent with the hypothesis that large males compete most intensively to monopolize mates.