Deployment dynamics analysis of CALLISTO’s approach and landing system

Prior to landing of reusable space transportation systems, the vehicle’s landing legs needs to be fully deployed to enable a safe landing and further re-use of the space vehicle. During that phase the deployment system has to overcome harsh and challenging environmental conditions. In this study, a numerical simulator is developed in order to investigate these influences on the landing leg deployment dynamics. By means of an extensive aerodynamic database and a broad approach flight domain, the influence of aerodynamics, exhaust plume, and vehicle’s attitude on the deployment dynamics is analyzed. This study shows on the example of the first stage demonstrator CALLISTO (Cooperative Action Leading to Launcher Innovation in Stage Toss back Operations), that thrust level, vehicle attitude, and the deployment system parameters affect the deployment performance.

A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract

The degree to which populations of cardiac progenitors (CPCs) persist in the postnatal heart remains a controversial issue in cardiobiology. To address this question, we conducted a spatiotemporally resolved analysis of CPC deployment dynamics, tracking cells expressing the pan-CPC gene Isl1. Most CPCs undergo programmed silencing during early cardiogenesis through proteasome-mediated and PRC2 (Polycomb group repressive complex 2)–mediated Isl1 repression, selectively in the outflow tract. A notable exception is a domain of cardiac neural crest cells (CNCs) in the inflow tract. These “dorsal CNCs” are regulated through a Wnt/β-catenin/Isl1 feedback loop and generate a limited number of trabecular cardiomyocytes that undergo multiple clonal divisions during compaction, to eventually produce ~10% of the biventricular myocardium. After birth, CNCs continue to generate cardiomyocytes that, however, exhibit diminished clonal amplification dynamics. Thus, although the postnatal heart sustains cardiomyocyte-producing CNCs, their regenerative potential is likely diminished by the loss of trabeculation-like proliferative properties.