Energetics of lipid transport by the ABC transporter MsbA is lipid dependent

The ABC multidrug exporter MsbA mediates the translocation of lipopolysaccharides and phospholipids across the plasma membrane in Gram-negative bacteria. Although MsbA is structurally well characterised, the energetic requirements of lipid transport remain unknown. Here, we report that, similar to the transport of small-molecule antibiotics and cytotoxic agents, the flopping of physiologically relevant long-acyl-chain 1,2-dioleoyl (C18)-phosphatidylethanolamine in proteoliposomes requires the simultaneous input of ATP binding and hydrolysis and the chemical proton gradient as sources of metabolic energy. In contrast, the flopping of the large hexa-acylated (C12-C14) Lipid-A anchor of lipopolysaccharides is only ATP dependent. This study demonstrates that the energetics of lipid transport by MsbA is lipid dependent. As our mutational analyses indicate lipid and drug transport via the central binding chamber in MsbA, the lipid availability in the membrane can affect the drug transport activity and vice versa.

High-Order Dual-Port Quasi-Absorptive Microstrip Coupled-Line Bandpass Filters

In this article, we present the first demonstration of distributed and symmetrical all-band quasi-absorptive filters that can be designed to arbitrarily high orders. The proposed quasi-absorptive filter consists of a bandpass section (reflective-type coupled-line filter) and absorptive sections (a matched resistor in series with a shorted quarter-wavelength transmission line). Through a detailed analysis, we show that the absorptive sections not only eliminate out-of-band reflections but also determine the passband bandwidth (BW). As such, the bandpass section mainly determines the out-of-band roll-off and the order of the filter can be arbitrarily increased without affecting the filter BW by cascading more bandpass sections. A set of 2.45-GHz one-, two-, and three-pole quasi-absorptive microstrip bandpass filters are designed and measured. The filters show simultaneous input and output absorption across both the passband and the stopband. Measurement results agree very well with the simulation and validate the proposed design concept.

High-Order Dual-Port Quasi-Absorptive Microstrip Coupled-Line Bandpass Filters

In this article, we present the first demonstration of distributed and symmetrical all-band quasi-absorptive filters that can be designed to arbitrarily high orders. The proposed quasi-absorptive filter consists of a bandpass section (reflective-type coupled-line filter) and absorptive sections (a matched resistor in series with a shorted quarter-wavelength transmission line). Through a detailed analysis, we show that the absorptive sections not only eliminate out-of-band reflections but also determine the passband bandwidth (BW). As such, the bandpass section mainly determines the out-of-band roll-off and the order of the filter can be arbitrarily increased without affecting the filter BW by cascading more bandpass sections. A set of 2.45-GHz one-, two-, and three-pole quasi-absorptive microstrip bandpass filters are designed and measured. The filters show simultaneous input and output absorption across both the passband and the stopband. Measurement results agree very well with the simulation and validate the proposed design concept.