alkaline phosphatase reaction
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scholarly journals Electrochemical Impedance Spectroscopy for Monitoring of Alkaline Phosphatase Reaction with Substrate

2017 ◽  
Vol 27 ◽  
pp. 315-316 ◽  
Author(s):  
Adriana Ferancova ◽  
Maarit Hattuniemi ◽  
Satu Pääkkönen ◽  
Pirkko Tervo ◽  
Elisa Ohtonen ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Alkaline Phosphatase Reaction

A revised mechanism for the alkaline phosphatase reaction involving three metal ions

2000 ◽  
Vol 299 (5) ◽  
pp. 1303-1311 ◽  
Author(s):  
Boguslaw Stec ◽  
Kathleen M. Holtz ◽  
Evan R. Kantrowitz
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Alkaline Phosphatase Reaction

scholarly journals A Model of the Transition State in the Alkaline Phosphatase Reaction

1999 ◽  
Vol 274 (13) ◽  
pp. 8351-8354 ◽  
Author(s):  
Kathleen M. Holtz ◽  
Boguslaw Stec ◽  
Evan R. Kantrowitz
Keyword(s):  
Alkaline Phosphatase ◽  
Transition State ◽  
Alkaline Phosphatase Reaction

scholarly journals Problems inherent in obtaining the alkaline phosphatase reaction.

1980 ◽  
Vol 28 (1) ◽  
pp. 66-68 ◽  
Author(s):  
S B Doty
Keyword(s):  
Electron Microscopy ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Enzyme Inhibitors ◽  
Light And Electron Microscopy ◽  
Histochemical Localization ◽  
Inorganic Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Alkaline Phosphatase Reaction

Problems encountered in the histochemical localization of alkaline phosphatase activity are discussed and solutions presented. The purpose is to achieve a reaction that can be studied by light and electron microscopy and to distinguish alkaline glycerophosphatase from inorganic pyrophosphatase activity. Details are presented concerning fixatives, fixation times, incubation media, enzyme inhibitors, activators, and associated techniques that can be used to obtain optimal histochemical results.

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