Detection of Mutations by Single-Strand Conformation Polymorphism (SSCP) Analysis and SSCP-Hybrid Methods

1997 ◽  
pp. 7.4.1-7.4.23 ◽  
Author(s):  
William Warren ◽  
Eivind Hovig ◽  
Birgitte Smith-Sørensen ◽  
Anne-Lise Børresen ◽  
Frank K. Fujimura ◽  
...  
Keyword(s):  
Hybrid Methods ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Sscp Analysis ◽  
Detection Of Mutations ◽  
Conformation Polymorphism

scholarly journals Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol

1997 ◽  
Vol 43 (1) ◽  
pp. 30-33 ◽  
Author(s):  
Arseni Markoff ◽  
Alex Savov ◽  
Vladimir Vladimirov ◽  
Nadia Bogdanova ◽  
Ivo Kremensky ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gc Content ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Sscp Analysis ◽  
Polymorphism Analysis ◽  
Conformation Polymorphism Analysis ◽  
Detection Of Mutations ◽  
Conformation Polymorphism

Abstract We report optimization of single-strand conformation polymorphism (SSCP) analysis in the presence of polyethylene glycol. The protocol developed separates single-strand conformers in a much shorter time (1–3 h) than conventional SSCP protocols and broadens the applicability of SSCP analysis from 150 to as much as 500 bp of DNA by different percentages of GC content present. We conclude that addition of polyethylene glycol helps improve the differential separation of conformers and, in combination with high-resolution polyacrylamide gel electrophoresis, offers an alternative to previous SSCP analysis protocols. This protocol should be very useful for clinical applications in routine detection of mutations as well as for research purposes.

Single-strand conformation polymorphism (SSCP) analysis ofListeria monocytogenes iapgene as tool to detect different serogroups

1997 ◽  
Vol 11 (6) ◽  
pp. 459-462 ◽  
Author(s):  
Marisa Manzano ◽  
Luca Cocolin ◽  
Corrado Pipan ◽  
Elisabetta Falasca ◽  
Guiseppe A Botta ◽  
...  
Keyword(s):  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Sscp Analysis ◽  
Oflisteria Monocytogenes ◽  
Conformation Polymorphism
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