3D-Gel Electrophoresis - A New Development in Protein Analysis

Author(s):  
Robert Ventzki ◽  
Josef Stegemann
Contraception ◽  
1988 ◽  
Vol 37 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Xue She-pu ◽  
Luo Hung-zi ◽  
Zhu Jia-bei ◽  
Li Hui-zhen ◽  
Zhao She-yun

2002 ◽  
Vol 48 (9) ◽  
pp. 787-792 ◽  
Author(s):  
Andrena M Kamp ◽  
Michael J Bidochka

Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on agar. Gel electrophoresis was used to examine differences in intracellular protein patterns (urea-soluble proteins and urea-insoluble proteins (i.e., hydrophobins)) in conidiating and mycelial cultures of M. anisopliae. Two major proteins present in a conidiating culture and one from a mycelial culture were N-terminally sequenced but showed no homologies to known proteins. The presence of hydrophobins in conidiating and mycelial cultures was also examined, and it was shown that these proteins were abundant in conidiating cultures but not in mycelial cultures. We also used primers designed from regulatory genes involved in conidiation in Aspergillus nidulans. The amplified fragments were not homologous to A. nidulans genes.Key words: Metarhizium anisopliae, conidia, pleomorphic deterioration, protein analysis.


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