Hydrothermal Deposition of Hydroxyapatite Coatings on Glass and Ceramics

Author(s):  
Haobo Pan ◽  
Mohamed N. Rahaman
Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 781 ◽  
Author(s):  
Paula E. Florian ◽  
Liviu Duta ◽  
Valentina Grumezescu ◽  
Gianina Popescu-Pelin ◽  
Andrei C. Popescu ◽  
...  

This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.


2017 ◽  
Vol 318 ◽  
pp. 39-49 ◽  
Author(s):  
Rolando T. Candidato ◽  
Paweł Sokołowski ◽  
Lech Pawłowski ◽  
Gisele Lecomte-Nana ◽  
Catalin Constantinescu ◽  
...  

2001 ◽  
Vol 72 (5) ◽  
pp. 613-618 ◽  
Author(s):  
J.M. Fernández-Pradas ◽  
L. Clèries ◽  
P. Serra ◽  
G. Sardin ◽  
J.L. Morenza

2001 ◽  
Vol 3 (8) ◽  
pp. 1153-1155 ◽  
Author(s):  
Miguel Manso ◽  
Michel Langlet ◽  
Carmen Jiménez ◽  
J.M Martı́nez-Duart

1994 ◽  
Vol 4 (12) ◽  
pp. 1849 ◽  
Author(s):  
G. Spoto ◽  
E. Ciliberto ◽  
G. C. Allen

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