scholarly journals 3D Printed Dual‐porosity Scaffolds: the Combined Effect of Stiffness and Porosity in the Modulation of Macrophage Polarization

2021 ◽  
pp. 2101415
Author(s):  
Sandra Camarero‐Espinosa ◽  
Maria Carlos Oliveira ◽  
Hong Liu ◽  
João F. Mano ◽  
Nicole Bouvy ◽  
...  
2006 ◽  
Vol 9 (05) ◽  
pp. 543-552 ◽  
Author(s):  
Carlos A. Pereira ◽  
Hossein Kazemi ◽  
Erdal Ozkan

Summary This paper addresses the combined effect of formation damage and non-Darcy flow in naturally fractured reservoirs using simplified analytical solutions and a 2D numerical simulator. Pressure drawdown, buildup, and isochronal tests simulated in this work indicate that, despite high fracture permeability, skin damage may accentuate the non-Darcy flow effect and drastically influence pressure-transient characteristics of low-pressure, naturally fractured reservoirs. In high-pressure reservoirs, this effect is significant only at high rates. Non-Darcy flow does not usually mask the typical pressure-transient characteristics of dual-porosity and dual-permeability reservoirs, but the conventional interpretation of the early-time data may lead to erroneous results. If the exponent, n, of the isochronal tests approaches 0.5 while the matrix permeability is low and flow rate is rather high, this would indicate the predominance of fracture flow. Under these conditions, small contributions from skin damage may greatly reduce gas-well performance in naturally fractured reservoirs. Introduction High velocity flow through porous media and fractures causes a higher pressure drop than predicted by the Darcy equation. This phenomenon, generally referred to as non-Darcy flow, was first described by Forchheimer (1901). Since then, it has been well established that the main variables that affect non-Darcy flow are the velocity, density, and saturation of the fluid and the permeability and porosity of the reservoir. Reservoir properties may be correlated to a single parameter, known as the non-Darcy flow coefficient, beta. Very little is known about the effect of other parameters, such as physical skin damage, on non-Darcy flow and their consequences in well performance. In fact, a recent literature review on non-Darcy flow by Li and Engler (2001a) indicates that most of the work has been focused on finding an accurate correlation for the non-Darcy flow coefficient, beta. There is also the issue of non-Darcy flow in dual-porosity and dual-permeability reservoirs, where high local velocities are prominent in the fractures. This paper pertains specifically to this issue. In general, the lower the formation permeability, the greater the non-Darcy pressure gradient. Formation damage in the near-wellbore region causes a drastic reduction in formation permeability, which potentially could be even more prominent in naturally fractured reservoirs. Thus, a greater non-Darcy flow effect could result in the wellbore region of a dual-porosity reservoir. The literature explaining the combined effect of physical damage and non-Darcy flow in single-porosity reservoirs is abundant (Berumen-C. et al. 1989; Camacho-V. et al. 1993; Fligelman et al. 1981); however, there is little information about such effects in dual-porosity and dual-permeability reservoirs. A finite-difference, 2D simulator in cylindrical coordinates was constructed to simulate pressure-drawdown and -buildup tests. By analyzing the simulated pressure drawdown and buildup tests, it was possible to decipher the combined effect of the skin damage and non-Darcy flow in fractured reservoirs. Both dual-porosity and dual-permeability idealizations of fractured reservoirs were considered.


2021 ◽  
Vol 10 (7) ◽  
pp. 411-424
Author(s):  
Da-Wang Zhao ◽  
Bing Ren ◽  
Hong-Wei Wang ◽  
Xiao Zhang ◽  
Ming-Zhi Yu ◽  
...  

Aims The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. Methods IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. Results DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. Conclusion DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration. Cite this article: Bone Joint Res 2021;10(7):411–424.


2007 ◽  
Vol 177 (4S) ◽  
pp. 79-80
Author(s):  
Jose A. Karam ◽  
Yair Lotan ◽  
Raheela Ashfaq ◽  
Claus G. Roehrborn ◽  
Arthur I. Sagalowsky ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document