In vivo ciliogenesis in human fetal tracheal epithelium

1989 ◽  
Vol 185 (4) ◽  
pp. 415-428 ◽  
Author(s):  
Dominique A. Gaillard ◽  
Aude V. Lallement ◽  
Annie F. Petit ◽  
Edith S. Puchelle
Keyword(s):  
Tracheal Epithelium

Features of Developing Ferret Tracheal Epithelium: Ultrastructural Observations of in Vivo and in Vitro Differentiation of Ciliated Cells

1987 ◽  
Vol 13 (2) ◽  
pp. 223-240 ◽  
Author(s):  
Linda N. Curtis ◽  
Johnny L. Carson ◽  
Albert M. Collier ◽  
Todd M. Gambling ◽  
S. S. Hu ◽  
...  
Keyword(s):  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
In Vitro Differentiation

In vivo differentiation potential of tracheal basal cells: evidence for multipotent and unipotent subpopulations

2004 ◽  
Vol 286 (4) ◽  
pp. L643-L649 ◽  
Author(s):  
Kyung U. Hong ◽  
Susan D. Reynolds ◽  
Simon Watkins ◽  
Elaine Fuchs ◽  
Barry R. Stripp
Keyword(s):  
Progenitor Cells ◽  
Progenitor Cell ◽  
Tracheal Epithelium ◽  
Secretory Cells ◽  
Basal Cells ◽  
Cell Type ◽  
Cell Hyperplasia ◽  
Differentiation Potential ◽  
Airway Injury

The composition of the conducting airway epithelium varies significantly along the proximal to distal axis, with that of the tracheal epithelium exhibiting the greatest complexity. A number of progenitor cells have been proposed to contribute to the maintenance of this cellular diversity both in the steady state and in response to injury. However, individual roles for each progenitor cell type are poorly defined in vivo. The present study was undertaken to investigate the hypothesis that basal cells represent a multipotent progenitor cell type for renewal of the injured tracheal epithelium. To understand their contribution to epithelial repair, mice were exposed to naphthalene to induce airway injury and depletion of the secretory cell progenitor pool. Injury resulted in a rapid induction of cytokeratin 14 (K14) expression among the majority of GSI-B4-reactive cells and associated hyperplasia of basal cells. Restoration of depleted secretory cells occurred after 6 days of recovery and was associated with regression of the basal cell hyperplasia, suggesting a progenitor-progeny relationship. Multipotent differentiation of basal cells was confirmed using a bitransgenic ligand-regulated Cre-loxP reporter approach in which expression of a ubiquitously expressed LacZ reporter was activated within K14-expressing progenitor cells during airway repair. With the use of this approach, it was determined that K14-expressing cells include subsets capable of either multipotent or unipotent differentiation in vivo. We conclude that basal cells have the capacity for restoration of a fully differentiated epithelium.

Effects of amphotericin B on ion and fluid movement across dog tracheal epithelium

1983 ◽  
Vol 55 (4) ◽  
pp. 1257-1261 ◽  
Author(s):  
I. Nathanson ◽  
J. H. Widdicombe ◽  
J. A. Nadel
Keyword(s):  
Amphotericin B ◽  
Short Circuit ◽  
Open Circuit ◽  
Tracheal Epithelium ◽  
Fluid Movement ◽  
Ussing Chambers ◽  
Cl Secretion ◽  
Special Apparatus ◽  
Net Fluxes

Ion fluxes or fluid flow were measured across sheets of dog tracheal epithelium mounted in Ussing chambers or a special apparatus, respectively. Under short-circuit conditions, luminal amphotericin B (3 X 10(-5) M) caused an inhibition of net Cl secretion and an increase in net Na absorption across paired tissues. In paired tissues under resting open-circuit conditions, there was no significant net transepithelial flux of either Cl or Na. Amphotericin B induced significant net fluxes of both Cl and Na toward the serosal side. In separate tissues from the same animals, there was no significant transepithelial fluid movement under resting conditions. Amphotericin B caused a net absorption of fluid. The absorption of salt and fluid in amphotericin B-treated tissues was abolished by ouabain. We conclude that stimulation of active Na transport by amphotericin B leads to fluid absorption. In vivo, the movement of fluid across the dog tracheal epithelium may be dependent on a balance between active Cl secretion and active Na absorption.

Ultrastructural studies of hamster tracheal epithelium in vivo and in vitro

1980 ◽  
Vol 70 (1) ◽  
pp. 70-81 ◽  
Author(s):  
Johnny L. Carson ◽  
Albert M. Collier ◽  
Shih-Chin S. Hu
Keyword(s):  
Tracheal Epithelium ◽  
Ultrastructural Studies

scholarly journals MHC class II antigen-bearing dendritic cells in pulmonary tissues of the rat. Regulation of antigen presentation activity by endogenous macrophage populations.

1988 ◽  
Vol 167 (2) ◽  
pp. 262-274 ◽  
Author(s):  
P G Holt ◽  
M A Schon-Hegrad ◽  
J Oliver
Keyword(s):  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Mononuclear Cells ◽  
Inhalation Exposure ◽  
Cell Activity ◽  
Tracheal Epithelium ◽  
Immunoperoxidase Staining ◽  
Tissue Slices

Collagenase digestion of tissue slices from perfused, lavaged SPF rat lung released approximately 10(8) viable mononuclear cells per gram tissue, which comprised 35% T lymphocytes and up to 26% macrophages. A subset of these cells that were Ia+, surface Ig-, nonadherent, FcR- and of ultra low density (putative dendritic cells [DC]), presented protein antigen to immune T cells in vitro, and this function was inhibited by the presence of low numbers of endogenous adherent, FcR+ cells (putative macrophages). APCs were also identified in digests from tracheal epithelium, and were shown to bind antigen in immunogenic form as a result of natural (inhalation) exposure in vivo. Immunoperoxidase staining of frozen sections revealed populations of strongly Ia+ cells with prominent DC-like morphology within the alveolar septal walls and the tracheal epithelium; in both areas, they were closely associated with pleiomorphic cells that expressed macrophage surface markers. We accordingly postulate that interactions between Ia+ antigen-presenting DCs and endogenous tissue macrophages play an important role in regulating T cell activity in the respiratory tract.

Decreased ion transport by tracheal epithelium of the basenji-greyhound dog

1994 ◽  
Vol 76 (4) ◽  
pp. 1489-1493 ◽  
Author(s):  
T. L. Croxton ◽  
M. Takahashi ◽  
C. A. Hirshman
Keyword(s):  
Ion Transport ◽  
Short Circuit ◽  
Airway Epithelial Cells ◽  
Tracheal Epithelium ◽  
Airway Epithelial ◽  
Beta Adrenergic ◽  
Electrical Potentials ◽  
Short Circuit Currents

The Basenji-Greyhound (BG) dog model shows altered beta-adrenergic function in both airway smooth muscle and leukocytes. To investigate a possible beta-adrenergic pathway defect in airway epithelial cells of BG dogs, we studied the electrophysiological behavior of tracheal epithelia in vitro and measured tracheal electrical potentials in vivo. Baseline short-circuit currents of isolated tracheal epithelia from BG (n = 6) and mongrel control dogs (n = 7) were 18.7 +/- 3.4 and 43.7 +/- 4.2 microA/cm2, respectively (P = 0.001). Significant differences between short-circuit currents of BG and control epithelia persisted after inhibition of Cl- secretion by indomethacin or stimulation by isoproterenol or dibutyryl adenosine 3',5'-cyclic monophosphate. In vivo tracheal potentials were also significantly less (P = 0.01) in BG dogs (-22.3 +/- 2.5 mV; n = 12) than in control dogs (-32.5 +/- 2.6 mV; n = 10), and intravenous indomethacin reduced the tracheal potential of BG dogs but had no effect in control animals. There was no correlation in BG dogs between tracheal potential and the dose of methacholine required to double total lung resistance. These data suggest that ion transport by tracheal epithelium is decreased in BG dogs, that this difference is not due to diminished beta-adrenergic activity, and that cyclooxygenase products are important in maintaining tracheal potential in vivo in this model.

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