Site-Selective Labeling of a Lysine Residue in Human Serum Albumin

2014 ◽  
Vol 126 (44) ◽  
pp. 11977-11980 ◽  
Author(s):  
Shigehiro Asano ◽  
James T. Patterson ◽  
Thomas Gaj ◽  
Carlos F. Barbas
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Lysine Residue ◽  
Selective Labeling ◽  
Site Selective

Site-Selective Labeling of a Lysine Residue in Human Serum Albumin

2014 ◽  
Vol 53 (44) ◽  
pp. 11783-11786 ◽  
Author(s):  
Shigehiro Asano ◽  
James T. Patterson ◽  
Thomas Gaj ◽  
Carlos F. Barbas
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Lysine Residue ◽  
Selective Labeling ◽  
Site Selective

scholarly journals Lysine residue 240 of human serum albumin is involved in high-affinity binding of bilirubin

1978 ◽  
Vol 171 (2) ◽  
pp. 453-459 ◽  
Author(s):  
C Jacobsen
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Lysine Residue ◽  
Water Soluble ◽  
High Affinity ◽  
Coupling Reagent ◽  
Peptide Fraction ◽  
Short Period ◽  
High Affinity Site

Bilirubin can be coupled covalently to albumin by using water-soluble carbodi-imide as coupling reagent. The optimal specificity in the attachment of bilirubin to the high-affinity site on the albumin molecule was obtained by treating an albumin-bilirubin complex with carbodi-imide in low concentrations and for a short period. The product was reduced, carboxymethylated and digested with trypsin. By fractionation on Sephadex G-50 (superfine grade) a peptide fraction containing most of the bilirubin label was isolated. Further purification by paper chromatography gave one peptide, consisting of residues 240-258. The peptide containined a single lysine residue, 240, and had an intact disulphide bridge. The results indicate that bilirubin is bound to lysine residue 240 at its high-affinity site on human serum albumin.

Evaluating the potential risk by probing the site-selective binding of rutin-Pr(III) complex to human serum albumin

2021 ◽  
Vol 148 ◽  
pp. 111927
Author(s):  
Chunlei Zhu ◽  
Fengru Liu ◽  
Yunlong Wei ◽  
Fan Zhang ◽  
Ting Pan ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Potential Risk ◽  
Selective Binding ◽  
Site Selective

Site-Selective Binding of Human Serum Albumin by Palmatine: Spectroscopic Approach

2010 ◽  
Vol 11 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Yan-Jun Hu ◽  
Yu Ou-Yang ◽  
Chun-Mei Dai ◽  
Yi Liu ◽  
Xiao-He Xiao
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Selective Binding ◽  
Site Selective

Investigating the site selective binding of busulfan to human serum albumin: Biophysical and molecular docking approaches

2018 ◽  
Vol 107 ◽  
pp. 1414-1421 ◽  
Author(s):  
Mohammad Siddiqi ◽  
Saima Nusrat ◽  
Parvez Alam ◽  
Sadia Malik ◽  
Sumit Kumar Chaturvedi ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Selective Binding ◽  
Site Selective
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