A Homonuclear Rotational Echo Double-Resonance Method for Measuring Site-Resolved Distance Distributions in I=1/2 Spin Pairs, Clusters, and Multispin Systems

2012 ◽  
Vol 51 (51) ◽  
pp. 12888-12891 ◽  
Author(s):  
Jinjun Ren ◽  
Hellmut Eckert
Keyword(s):  
Double Resonance ◽  
Resonance Method ◽  
Distance Distributions ◽  
Rotational Echo Double Resonance ◽  
Measuring Site ◽  
Spin Pairs ◽  
Multispin Systems

Pulsed electron–electron double resonance: beyond nanometre distance measurements on biomacromolecules

2011 ◽  
Vol 434 (3) ◽  
pp. 353-363 ◽  
Author(s):  
Gunnar W. Reginsson ◽  
Olav Schiemann
Keyword(s):  
Protein Complexes ◽  
Double Resonance ◽  
Resonance Method ◽  
Spin Labels ◽  
Coupling Mechanism ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Distance Distributions ◽  
Nitroxide Spin Labels ◽  
Amino Acid Radicals

PELDOR (or DEER; pulsed electron–electron double resonance) is an EPR (electron paramagnetic resonance) method that measures via the dipolar electron–electron coupling distances in the nanometre range, currently 1.5–8 nm, with high precision and reliability. Depending on the quality of the data, the error can be as small as 0.1 nm. Beyond mere mean distances, PELDOR yields distance distributions, which provide access to conformational distributions and dynamics. It can also be used to count the number of monomers in a complex and allows determination of the orientations of spin centres with respect to each other. If, in addition to the dipolar through-space coupling, a through-bond exchange coupling mechanism contributes to the overall coupling both mechanisms can be separated and quantified. Over the last 10 years PELDOR has emerged as a powerful new biophysical method without size restriction to the biomolecule to be studied, and has been applied to a large variety of nucleic acids as well as proteins and protein complexes in solution or within membranes. Small nitroxide spin labels, paramagnetic metal ions, amino acid radicals or intrinsic clusters and cofactor radicals have been used as spin centres.

Quantitative Rotational-Echo Double Resonance for Carbon-13 Spin Clusters

2021 ◽  
pp. 107043
Author(s):  
Shigeru Matsuoka ◽  
Miriam Sindelar ◽  
Sonal Bansal ◽  
Gary J. Patti ◽  
Jacob Schaefer
Keyword(s):  
Double Resonance ◽  
Rotational Echo Double Resonance ◽  
Spin Clusters

In SituAnalysis of Peptidyl DOPA in Mussel Byssus Using Rotational-Echo Double-Resonance NMR

1996 ◽  
Vol 333 (1) ◽  
pp. 221-224 ◽  
Author(s):  
Christopher A. Klug ◽  
Luis A. Burzio ◽  
J.Herbert Waite ◽  
Jacob Schaefer
Keyword(s):  
Double Resonance ◽  
Rotational Echo Double Resonance ◽  
Mussel Byssus

Structural Constraints on the Ternary Complex of 5-Enolpyruvylshikimate-3-phosphate Synthase from Rotational-echo Double-resonance NMR

1996 ◽  
Vol 256 (1) ◽  
pp. 160-171 ◽  
Author(s):  
Lynda M. McDowell ◽  
Asher Schmidt ◽  
Eric R. Cohen ◽  
Daniel R. Studelska ◽  
Jacob Schaefer
Keyword(s):  
Ternary Complex ◽  
Double Resonance ◽  
Structural Constraints ◽  
Rotational Echo Double Resonance ◽  
Phosphate Synthase

scholarly journals Double Resonance Method for Determination of Gel Point

2019 ◽  
pp. 160-165
Author(s):  
Ivan S. Kozhevnikov ◽  
Lyubov K. Altunina ◽  
Andrey V. Bogoslovsky ◽  
Lyubov A. Stasyeva
Keyword(s):  
Double Resonance ◽  
Resonance Method ◽  
Gel Point ◽  
Cellulose Ether ◽  
Different Diameters

The application of double resonance method for determining the dynamics of fluidity changes of thermotropic gel-forming compositions based on cellulose ether is considered. To determine the gel point, a modified version of the ‘Rheokinetika’ viscometer including two identical sensors and measuring cells of different diameters

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