Effects of concentration of media and elevated temperature on cell division in tissue cultures of chick tissue

1957 ◽  
Vol 127 (1) ◽  
pp. 15-29 ◽  
Author(s):  
E. Frances Stilwell
Keyword(s):  
Cell Division ◽  
Elevated Temperature ◽  
Tissue Cultures ◽  
Chick Tissue

The Effect of Inhibitory Substances on Cell Division

1950 ◽  
Vol s3-91 (15) ◽  
pp. 251-277
Author(s):  
A.F. W. HUGHES
Keyword(s):  
Cell Division ◽  
Phase Contrast ◽  
Tissue Cultures ◽  
Cell Divisions ◽  
High Concentrations ◽  
Chick Tissue ◽  
Very High ◽  
The Way

1. The effect of inhibitory substances upon cell divisions in chick tissue cultures has been, studied mainly by phase-contrast cine-micrography. 2. Fluoride and powerful -SH reactants prevent the entry of cells into prophase within I hour. 3. Aminopterin and colchicine are complete spindle inhibitors; they prevent its formation at the end of prophase, and can act on all metaphase cells in the culture. 4. The effect of fluoride, cyanide, and urethane on the spindle is much less drastic. 5. Most substances can retard nuclear reconstruction in telophase to some extent, but only chloracetophenone acts in the way already described for iodoacetamide. 6. Malonate appears to have no effect on mitosis, and fluoracetate only at very high concentrations. 7. Possible interpretations of some of these results are discussed.

scholarly journals Activity and Accumulation of Cell Division-Promoting Phenolics in Tobacco Tissue Cultures

1991 ◽  
Vol 97 (1) ◽  
pp. 288-297 ◽  
Author(s):  
Rita A. Teutonico ◽  
Matthew W. Dudley ◽  
John D. Orr ◽  
David G. Lynn ◽  
Andrew N. Binns
Keyword(s):  
Cell Division ◽  
Tissue Cultures

Restoration of cell division in tissue cultures after cooling

1968 ◽  
Vol 66 (3) ◽  
pp. 1023-1025 ◽  
Author(s):  
V. V. Portugalov ◽  
F. V. Sushkov ◽  
V. B. Starikova
Keyword(s):  
Cell Division ◽  
Tissue Cultures

Promotion of Cell Division in Tissue Cultures by Gibberellic Acid

Nature ◽  
1964 ◽  
Vol 203 (4944) ◽  
pp. 547-548 ◽  
Author(s):  
J. DIGBY ◽  
T. H. THOMAS ◽  
P. F. WAREING
Keyword(s):  
Cell Division ◽  
Gibberellic Acid ◽  
Tissue Cultures

Correlation between rate of cell division and loss of embryogenesis in longterm tissue cultures

PROTOPLASMA ◽  
1973 ◽  
Vol 78 (3) ◽  
pp. 273-283 ◽  
Author(s):  
H. Meyer-Teuter ◽  
J. Reinert
Keyword(s):  
Cell Division ◽  
Tissue Cultures

scholarly journals The effect of gamma irradiation on cell division in tissue culture in vitro .—Part II

1929 ◽  
Vol 105 (734) ◽  
pp. 93-98 ◽  
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
X Rays ◽  
Tissue Cultures ◽  
Long Period ◽  
Silk Worm ◽  
Number Of Cells

In a previous paper (1) the effects of the gamma rays of radium upon the number of cells in mitosis in tissue cultures has been described, the cultures being examined 80 minutes after irradiation. Under the conditions of the experiment it was shown that there was a threshold of intensity below which no diminution in the number of cells in mitosis was apparent, and also a threshold of time for each intensity which must be exceeded before diminution could be observed. Gilman and Baetjer (2) showed that there was an acceleration in the development of the eggs of Amblystoma after irradiation by X-rays. Hastings, Beckton and Wedd (4) showed an increase in the rate of hatching out of silk-worm eggs which had been irradiated by X-rays; and Lazarus-Barlow and Beckton (3) showed that small intensities of beta-rays acting for a long period were followed by a greater rate of cell division in the eggs of Ascaris Megalocephala . In the case of tissue cultures Canti and Donaldson (5) described an experiment in which cessation of mitosis having been caused by exposure to the gamma rays of radium a return of mitosis was observed after removal of the radium. Since the completion of the present experiments it has been shown by Spear (6) that by lowering the temperature of tissue cultures to 0° C. for 4 hours and subsequently incubating for various periods there is a fall in the number of cells in mitosis followed by a return in increased numbers which is compensatory.

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