Growth and Differentiation of Plant Tissue Cultures III. The Initiation and Pattern of Cell Division in Developing Callus Cultures

1968 ◽  
Vol 32 (2) ◽  
pp. 301-313 ◽  
Author(s):  
M. M. YEOMAN ◽  
G. G. NAIK ◽  
A. I. ROBERTSON
Keyword(s):  
Cell Division ◽  
Plant Tissue ◽  
Callus Cultures ◽  
Tissue Cultures ◽  
Plant Tissue Cultures

scholarly journals Production of Podophyllotoxin by Plant Tissue Cultures of Juniperus virginiana

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Marie Kašparová ◽  
Jan Martin ◽  
Lenka Tůmová ◽  
Jiřina Spilková
Keyword(s):  
Plant Tissue ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Juniperus Virginiana ◽  
Tissue Cultures ◽  
Potential Source ◽  
Plant Tissue Cultures ◽  
One Year ◽  
Maximum Content ◽  
Biogenetic Precursor

Plant tissue cultures are a potential source of secondary metabolites. However, their production, when compared with intact plants, is usually lower. Phenylalanine, a biogenetic precursor of podophyllotoxin, was used to stimulate podophyllotoxin production in callus and suspension cultures of Juniperus virginiana L. The best phenylalanine effect on podophyllotoxin production was manifested in three-years-old callus cultures after a 21-days application of a 10 mmol/L concentration. A podophyllotoxin content of 0.15 mg/g DW was determined, which was about 400% higher in comparison with the control. The maximum content (0.48 mg/g DW) in newly derived suspension cultures (the 4th passage) was induced by 14-days application of a 1 mmol/L concentration; this was about 243% higher than the control. In one-year-old suspension cultures the highest podophyllotoxin content (0.56 mg/g DW) was recorded also after 14-days application of a 1 mmol/L concentration; this was about 211% higher than in the control cultures.

scholarly journals Effect of Light on Cell Division in Plant Tissue Cultures

Nature ◽  
1967 ◽  
Vol 215 (5103) ◽  
pp. 873-873 ◽  
Author(s):  
R. S. S. FRASER ◽  
U. E. LOENING ◽  
M. M. YEOMAN
Keyword(s):  
Cell Division ◽  
Plant Tissue ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
Effect Of Light

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

Cold acclimation of plant tissue cultures

Cryobiology ◽  
1971 ◽  
Vol 8 (4) ◽  
pp. 386-387 ◽  
Author(s):  
Peter L. Steponkus ◽  
L. Bannier
Keyword(s):  
Cold Acclimation ◽  
Plant Tissue ◽  
Tissue Cultures ◽  
Plant Tissue Cultures

Iron Nutrition for Growth and Chlorophyll Development of some Plant Tissue Cultures

Nature ◽  
1964 ◽  
Vol 202 (4938) ◽  
pp. 1235-1236 ◽  
Author(s):  
J. C. WILMAR ◽  
A. C. HILDEBRANDT ◽  
A. J. RIKER
Keyword(s):  
Plant Tissue ◽  
Iron Nutrition ◽  
Tissue Cultures ◽  
Plant Tissue Cultures
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