Glucose‐6‐phosphate dehydrogenase is posttranslationally regulated in the larvae of the freeze‐tolerant gall fly, Eurosta solidaginis , in response to freezing

2019 ◽  
Vol 102 (4) ◽  
Author(s):  
Michael B. Smolinski ◽  
Stuart R. Green ◽  
Kenneth B. Storey
Keyword(s):  
Eurosta Solidaginis ◽  
Freeze Tolerant ◽  
Glucose 6 Phosphate Dehydrogenase

Ultrastructural effects of lethal freezing on brain, muscle and Malpighian tubules from freeze-tolerant larvae of the gall fly, Eurosta solidaginis

1997 ◽  
Vol 43 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Stephen D Collins ◽  
Allan L Allenspach ◽  
Richard E Lee
Keyword(s):  
Malpighian Tubules ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

Modeling seasonal changes in intracellular freeze-tolerance of fat body cells of the gall fly Eurosta solidaginis (Diptera, Tephritidae)

1997 ◽  
Vol 200 (1) ◽  
pp. 185-192 ◽  
Author(s):  
V Bennett ◽  
R Lee
Keyword(s):  
Cell Survival ◽  
Seasonal Changes ◽  
Cold Hardiness ◽  
Fat Body ◽  
Freeze Tolerance ◽  
Cellular Level ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

Although seasonal changes in the freeze-tolerance of third-instar larvae of Eurosta solidaginis have been well documented for the whole organism, the nature of this cold-hardiness at the cellular level has not been examined. Seasonal changes in the survival of fat body cells from E. solidaginis larvae were assessed using fluorescent vital dyes after freezing at -10, -25 or -80 °C for 24 h both in vivo and in vitro. Cells frozen in vitro were frozen with glycerol, with sorbitol (both of which enhanced cell survival) or without cryoprotectants. Both cellular and organismal survival were low in August when larvae were not freeze-tolerant, then increased dramatically during September and October before leveling off from November to January. This observation for cells frozen without cryoprotectants indicates that the cells themselves have adapted. The single most important factor influencing cell survival, as determined by logistic regression modeling, was the time of larval collection, which reflects the level of cold-hardiness achieved by field acclimation. Cells frozen in vivo exhibited greater survival than did those frozen in vitro, even with the addition of cryoprotectants. Since no differences were observed between cells frozen with glycerol or sorbitol, the role of the multi-component cryoprotectant system present in E. solidaginis should be investigated.

Oxidative stress and antioxidants in overwintering larvae of cold-hardy goldenrod gall insects

1996 ◽  
Vol 199 (7) ◽  
pp. 1483-1491 ◽  
Author(s):  
D Joanisse ◽  
K Storey
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Xanthine Oxidase ◽  
Xanthine Dehydrogenase ◽  
Oxygen Species ◽  
Eurosta Solidaginis ◽  
Epiblema Scudderiana ◽  
Freeze Tolerant ◽  
Cold Hardy ◽  
Gall Insect

Antioxidant and pro-oxidant systems were studied in overwintering larvae of two cold-hardy gall insect species, the freeze-tolerant fly Eurosta solidaginis and the freeze-avoiding moth Epiblema scudderiana. An increase in the levels of the oxidized form of glutathione suggested slight oxidative stress in both species during the winter. Freeze-tolerant Eurosta solidaginis larvae generally had decreased activities of antioxidant enzymes in the winter, indicating that these larvae do not face increased challenge from oxidative stress during the numerous freeze-thaw events they experience. Instead, existing defences must be sufficient to prevent any damage. By contrast, increased winter activities of antioxidant enzymes in freeze-avoiding Epiblema scudderiana suggest that these larvae must defend against the formation of reactive oxygen species. This may result from the oxidative nature of winter metabolism in these larvae, as well as a dependence on lipid oxidation as their fuel over this season. Xanthine dehydrogenase activity decreased dramatically in both species during the autumn, reducing the potential for the formation of the pro-oxidant xanthine oxidase. Indeed, xanthine oxidase activity fell to undetectable levels by winter in Epiblema scudderiana and was not detectable at any time in Eurosta solidaginis.

Survival of intracellular freezing, lipid coalescence and osmotic fragility in fat body cells of the freeze-tolerant gall fly Eurosta solidaginis

1993 ◽  
Vol 39 (5) ◽  
pp. 445-450 ◽  
Author(s):  
Richard E. Lee ◽  
John J. McGrath ◽  
R. Todd Morason ◽  
Ronald M. Taddeo
Keyword(s):  
Fat Body ◽  
Osmotic Fragility ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

Characterization of cold-associated microRNAs in the freeze-tolerant gall fly Eurosta solidaginis using high-throughput sequencing

2016 ◽  
Vol 20 ◽  
pp. 95-100 ◽  
Author(s):  
Pierre J. Lyons ◽  
Louise Govaere ◽  
Nicolas Crapoulet ◽  
Kenneth B. Storey ◽  
Pier Jr Morin
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

Differential expression of microRNA species in a freeze tolerant insect, Eurosta solidaginis

Cryobiology ◽  
2012 ◽  
Vol 65 (3) ◽  
pp. 210-214 ◽  
Author(s):  
Lynn A. Courteau ◽  
Kenneth B. Storey ◽  
Pier Jr. Morin
Keyword(s):  
Differential Expression ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

Expression of miRNAs in response to freezing and anoxia stresses in the freeze tolerant fly Eurosta solidaginis

Cryobiology ◽  
2015 ◽  
Vol 71 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Pierre J. Lyons ◽  
Kenneth B. Storey ◽  
Pier Jr. Morin
Keyword(s):  
Eurosta Solidaginis ◽  
Freeze Tolerant

Repeated freezing induces oxidative stress and reduces survival in the freeze-tolerant goldenrod gall fly, Eurosta solidaginis

2014 ◽  
Vol 67 ◽  
pp. 20-27 ◽  
Author(s):  
Adam R.W. Doelling ◽  
Nicole Griffis ◽  
Jason B. Williams
Keyword(s):  
Oxidative Stress ◽  
Eurosta Solidaginis ◽  
Repeated Freezing ◽  
Freeze Tolerant

scholarly journals Cryobiology of the freeze-tolerant gall fly Eurosta solidaginis: overwintering energetics and heat shock proteins

1995 ◽  
Vol 5 ◽  
pp. 61-67 ◽  
Author(s):  
RE Lee ◽  
RA Dommel ◽  
KH Joplin ◽  
DL Denlinger
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant ◽  
Shock Proteins

Evidence for a novel cryoprotective protein from freeze-tolerant larvae of the goldenrod gall fly Eurosta solidaginis

Cryobiology ◽  
2007 ◽  
Vol 54 (1) ◽  
pp. 125-128 ◽  
Author(s):  
Nancy L. Pruitt ◽  
Nasheed Moqueet ◽  
Craig A. Shapiro
Keyword(s):  
Eurosta Solidaginis ◽  
Freeze Tolerant
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