Analysis of carotenoid profile changes and carotenogenic genes transcript levels in Rhodosporidium toruloides mutants from an optimized Agrobacterium tumefaciens ‐mediated transformation method

2020 ◽  
Author(s):  
Zeping Sun ◽  
Jing Lv ◽  
Chaofan Ji ◽  
Huipeng Liang ◽  
Shengjie Li ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transformation Method ◽  
Rhodosporidium Toruloides ◽  
Transcript Levels ◽  
Carotenogenic Genes ◽  
Profile Changes ◽  
Carotenoid Profile

Tissue Culture Independent Agrobacterium tumefaciens Mediated In Planta Transformation Method for Tropical Maize (Zea mays.L)

2014 ◽  
Vol 86 (2) ◽  
pp. 375-384 ◽  
Author(s):  
Alok Abhishek ◽  
Rita Kumari ◽  
Chikkappa G. Karjagi ◽  
Pradyumn Kumar ◽  
Bhupender Kumar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Agrobacterium Tumefaciens ◽  
Transformation Method ◽  
In Planta Transformation ◽  
Tropical Maize ◽  
In Planta ◽  
Culture Independent

scholarly journals Establishment of an efficient genetic transformation method in Dunaliella tertiolecta mediated by Agrobacterium tumefaciens

2018 ◽  
Vol 150 ◽  
pp. 9-17 ◽  
Author(s):  
Claudia D. Norzagaray-Valenzuela ◽  
Lourdes J. Germán-Báez ◽  
Marco A. Valdez-Flores ◽  
Sergio Hernández-Verdugo ◽  
Luke M. Shelton ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Transformation Method ◽  
Dunaliella Tertiolecta ◽  
Efficient Genetic Transformation

Development of simple and efficient in Planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens

2006 ◽  
Vol 102 (3) ◽  
pp. 162-170 ◽  
Author(s):  
Putu Supartana ◽  
Tsutomu Shimizu ◽  
Masahiro Nogawa ◽  
Hidenari Shioiri ◽  
Tadashi Nakajima ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Agrobacterium Tumefaciens ◽  
Triticum Aestivum L ◽  
Transformation Method ◽  
In Planta Transformation ◽  
In Planta

scholarly journals Evaluasi varietas, sumber eksplan dan strain Agrobacterium terhadap keberhasilan transformasi tebu dengan gen P5CS Evaluation of varieties, explant sources, and Agrobacterium strains for successful sugarcane transformation using P5CS gene

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Hayati MINARSIH ◽  
Dwi SUBIYARTI ◽  
Imron RIYADI ◽  
Soekarno Mismana PUTRA ◽  
Laksmi AMBARSARI
Keyword(s):  
Drought Stress ◽  
Agrobacterium Tumefaciens ◽  
Somatic Embryos ◽  
Saccharum Officinarum ◽  
Transformation Method ◽  
Embryogenic Calli ◽  
Temporary Immersion System ◽  
Agrobacterium Strain ◽  
Solid Media ◽  
P5cs Gene

Abstract Genetic transformation can be used as an alter-native to develop sugarcane (Saccharum officinarum L.) tolerant to drought stress. P5CS gene has a role in biosynthesis of proline, an amino acid that accumulated under drought stress conditions. Transfer of a P5CS gene construct into plant cells in conjunction with regeneration of transgenic plantlets may develop sugarcane tolerant to drought stress. The aim of this research was to obtain an optimal transformation method which includes a suitable strain of Agrobacterium tumefaciens, and the best sugarcane explant and variety. The results showed that transfer of P5CS gene has been successfully carried out on sugarcane explants from solid media-derived calli, embryogenic calli and somatic embryos derived from temporary immersion system (TIS) culture. Whilst Agrobacterium strain LBA4404 was indicated as the most effective transformation vector. The regeneration of Kidang Kencana variety transformants from calli and somatic embryos was better than those of PS 881 and PS 891. The best performance of transformants based on the source of explants obtained from somatic embryos from TIS culture. Moreover, a succesfull Agrobacterium mediated transformation on sugarcane was indicated by transient expression of Gus gene and the ability of the transformants grew in a selection medium containing 50 ppm of kanamycin.Abstrak Transformasi genetik dapat digunakan sebagai upaya untuk merakit tebu (Saccharum officinarum L.) toleran terhadap cekaman kekeringan. Gen P5CS diketahui  berperan  dalam  biosintesis  prolin,  yaitu asam amino yang umumnya terakumulasi ketika tanaman mengalami cekaman kekeringan. Transfor-masi gen P5CS dan regenerasi transgeniknya mungkin dapat menghasilkan tanaman tebu trans-genik yang toleran terhadap cekaman kekeringan. Tujuan penelitian ini adalah untuk mendapatkan metode transformasi yang optimum yang mencakup strain Agrobacterium tumefaciens yang sesuai, sumber eksplan dan varietas tebu terbaik sebagai target transformasi. Hasil penelitian menunjukkan bahwa transformasi gen P5CS telah berhasil dilakukan ke eksplan tebu baik yang berupa kalus asal media padat maupun kalus embriogenik dan embrio somatik asal kultur sistem perendaman sesaat (SPS). Sementara itu strain A. tumefaciens LBA4404 menunjukkan hasil yang paling efektif sebagai vektor transformasi. Pertumbuhan transforman baik pada kalus maupun embrio somatik pada varietas Kidang Kencana terlihat paling baik dibandingkan dengan varietas PS 881 dan PS 891. Sumber eksplan yang paling efektif adalah embrio somatik yang diperoleh dari  kultur SPS. Keberhasilan transformasi tebu me-lalui Agrobacterium ditunjukkan oleh ekspresi transien dari gen GUS dan kemampuan dari trans-forman untuk tumbuh di media yang mengandung    50 ppm kanamisin.

scholarly journals An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

Mycobiology ◽  
2019 ◽  
Vol 47 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Yuanyuan Wang ◽  
Danyun Xu ◽  
Xueyan Sun ◽  
Lisheng Zheng ◽  
Liguo Chen ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transformation Method ◽  
Tremella Fuciformis

A simple and efficient in planta transformation method for pommelo ( Citrus maxima ) using Agrobacterium tumefaciens

2017 ◽  
Vol 214 ◽  
pp. 174-179 ◽  
Author(s):  
Yong-yan Zhang ◽  
Dong-min Zhang ◽  
Yun Zhong ◽  
Xiao-jun Chang ◽  
Min-lun Hu ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transformation Method ◽  
In Planta Transformation ◽  
In Planta ◽  
Citrus Maxima

scholarly journals An efficient Agrobacterium tumefaciens-mediated transformation method for Simplicillium subtropicum (Hypocreales: Cordycipitaceae)

2021 ◽  
Vol 44 (3) ◽  
Author(s):  
Nicolau Sbaraini ◽  
Mariana Vieira Tomazett ◽  
Augusto Bartz Penteriche ◽  
Relber Aguiar Gonçales ◽  
Matheus da Silva Camargo ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transformation Method
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