Biotechnological production of xylitol in a three-phase fluidized bed bioreactor with immobilized yeast cells in Ca-alginate beads

2007 ◽  
Vol 2 (6) ◽  
pp. 759-763 ◽  
Author(s):  
Boutros Fouad Sarrouh ◽  
Diego Tresinari dos Santos ◽  
Silvio Silvério da Silva
2010 ◽  
Vol 16 (2) ◽  
pp. 133-137
Author(s):  
Dragan Povrenovic ◽  
Viktor Nedovic

Brewery yeast cells immobilized in Ca-alginate were dried in a laboratory scale spouted bed with a draft tube The experiment was conducted under variable temperatures and air flow rates. Temperature and air velocity at the bottom of the column has been varied in the range from 30 to 60 oC and from 6 to 10 m/s in a duration of 60 minutes. The moisture of dryied particles was in the interval of 10.00 to 21.00 g/g , while the water activity was in the range of 0.40 to 0.45, what ensures preservation of immobilized yeast as a starter and provides the biological activity of dried particles. Rehidration process of dryied particles, proved that dried particles could restore completely their original shape and starting volume, while the mechanical resistance is somewhat reduced. The cells preserved in this way after rehidration completely restore their catalytical activity.


2000 ◽  
Vol 177 (1) ◽  
pp. 1-14 ◽  
Author(s):  
YOSHIMITSU UEMURA ◽  
NAOKl HAMAKAWA ◽  
HIDEKAZU YOSHIZAWA ◽  
HIROKI ANDO ◽  
KAZUYA IJICHIY ◽  
...  

Author(s):  
Andri Cahyo Kumoro ◽  
Astrilia Damayanti ◽  
Zuhriyan Ash Shiddieqy Bahlawan ◽  
Mira Melina ◽  
Heti Puspawati

Bioethanol is an environmentally benign renewable energy commonly obtained from glucose fermentation using Saccharomyces cerevisiae. The purposes of this study are to investigate the effects of time, temperature, pH, immobilized yeast cell loading, beads reuse during ethanol production through batch fermentation of glucose derived from oil palm empty fruit bunches by S. cerevisiae immobilized on Na-alginate beads and to compare the performance of fermentation using immobilized yeast cells and that of using a free cell system. The results revealed that time, temperature, pH, yeast mass and beads reuse significantly affected the ethanol and final glucose concentrations. As expected, a maximum ethanol concentration was obtained from fermentation using immobilized yeast cells at 30 °C, pH 5, and immobilized yeast cell loading of 0.75 g for 48 hours. However, fermentation with a free cell system at the same conditions resulted in lower ethanol yield. The highest ethanol concentration of 88.125 g/L with a productivity of 1.84 g/L·h was achieved from the second cycle fermentation using of immobilized cells beads. The results suggest that an immobilized cell system exhibits great potential applications for improved ethanol production due to its ability to sustain the stability of cell activity, reduce contamination tendency, and protect yeast cells from any possible inhibitions.


2016 ◽  
Vol 75 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Yiang Fan ◽  
Yunhai Wu ◽  
Peng Fang ◽  
Zhu Ming

We compared the adsorption efficiency of phosphates onto Ca-alginate immobilized yeast and freely suspended yeast under different conditions of pH and temperature. The results clearly demonstrated that the adsorption efficiency onto Ca-alginate immobilized yeast was better than that of freely suspended yeast, and reached a maximum at pH 9.17 and 35 °C. Scanning electron microscopy was used to characterize the morphology of Ca-alginate immobilized yeast. Fitting the adsorption equilibrium data to existing models showed that the Freundlich isotherm model described the process better than the Langmuir model, and the process of adsorption followed pseudo-first-order kinetics. During the initial period of experiment, external diffusion was a key rate-controlling step, and intraparticle diffusion also contributed to the mass transport. The thermodynamic properties (Gibbs free energy change of −15.143 kJ/mol, enthalpy change of 274.118 kJ/mol, and entropy change of 290 J/(mol K)) indicated that the adsorption process was endothermic.


2009 ◽  
Vol 33 (1) ◽  
pp. 359-370 ◽  
Author(s):  
Mauren Fuentes ◽  
Miguel C. Mussati ◽  
Nicolás J. Scenna ◽  
Pío A. Aguirre

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