scholarly journals Metabolic Engineering of Corynebacterium glutamicum for High‐Level Ectoine Production: Design, Combinatorial Assembly, and Implementation of a Transcriptionally Balanced Heterologous Ectoine Pathway

2019 ◽  
Vol 14 (9) ◽  
pp. 1800417 ◽  
Author(s):  
Gideon Gießelmann ◽  
Demian Dietrich ◽  
Lukas Jungmann ◽  
Michael Kohlstedt ◽  
Eun J. Jeon ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
Combinatorial Assembly ◽  
High Level

Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510

2018 ◽  
Vol 6 (4) ◽  
pp. 5296-5305 ◽  
Author(s):  
Hee Taek Kim ◽  
Kei-Anne Baritugo ◽  
Young Hoon Oh ◽  
Sung Min Hyun ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
High Level ◽  
Level Production

Fermentative High-Level Production of 5-Hydroxyvaleric Acid by Metabolically Engineered Corynebacterium glutamicum

2021 ◽  
Vol 9 (6) ◽  
pp. 2523-2533
Author(s):  
Yu Jung Sohn ◽  
Minsoo Kang ◽  
Kei-Anne Baritugo ◽  
Jina Son ◽  
Kyoung Hee Kang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Hydroxyvaleric Acid ◽  
High Level ◽  
Level Production

scholarly journals RamB, a Novel Transcriptional Regulator of Genes Involved in Acetate Metabolism of Corynebacterium glutamicum

2004 ◽  
Vol 186 (9) ◽  
pp. 2798-2809 ◽  
Author(s):  
Robert Gerstmeir ◽  
Annette Cramer ◽  
Petra Dangel ◽  
Steffen Schaffer ◽  
Bernhard J. Eikmanns
Keyword(s):  
Corynebacterium Glutamicum ◽  
Isocitrate Lyase ◽  
Transcriptional Regulator ◽  
Regulatory Elements ◽  
Malate Synthase ◽  
Acetate Kinase ◽  
Acetate Metabolism ◽  
Peptide Mass ◽  
Specific Activities ◽  
High Level

ABSTRACT The adaptation of Corynebacterium glutamicum to acetate as a carbon and energy source involves transcriptional regulation of the pta-ack operon coding for the acetate-activating enzymes phosphotransacetylase and acetate kinase and of the aceA and aceB genes coding for the glyoxylate cycle enzymes isocitrate lyase and malate synthase, respectively. Deletion and mutation analysis of the respective promoter regions led to the identification of highly conserved 13-bp motifs (AA/GAACTTTGCAAA) as cis-regulatory elements for expression of the pta-ack operon and the aceA and aceB genes. By use of DNA affinity chromatography, a 53-kDa protein specifically binding to the promoter/operator region of the pta-ack operon was purified. Mass spectrometry and peptide mass fingerprinting identified the protein as a putative transcriptional regulator (which was designated RamB). Purified His-tagged RamB protein was shown to bind specifically to both the pta-ack and the aceA/aceB promoter/operator regions. Directed deletion of the ramB gene in the genome of C. glutamicum resulted in mutant strain RG1. Whereas the wild type of C. glutamicum showed high-level specific activities of acetate kinase, phosphotransacetylase, isocitrate lyase, and malate synthase when grown on acetate and low-level specific activities when grown on glucose as sole carbon and energy sources, mutant RG1 showed high-level specific activities with all four enzymes irrespective of the substrate. Comparative transcriptional cat fusion experiments revealed that this deregulation takes place at the level of transcription. The results indicate that RamB is a negative transcriptional regulator of genes involved in acetate metabolism of C. glutamicum.

An improved shuttle vector constructed for metabolic engineering research in Corynebacterium glutamicum

Plasmid ◽  
2010 ◽  
Vol 64 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Daqing Xu ◽  
Yanzhen Tan ◽  
Feng Shi ◽  
Xiaoyuan Wang
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
Shuttle Vector ◽  
Engineering Research

Combining Protein and Metabolic Engineering Strategies for High-Level Production of O-Acetylhomoserine in Escherichia coli

2019 ◽  
Vol 8 (5) ◽  
pp. 1153-1167 ◽  
Author(s):  
Liang Wei ◽  
Qian Wang ◽  
Ning Xu ◽  
Jian Cheng ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
High Level ◽  
Level Production
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