Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510

2018 ◽  
Vol 6 (4) ◽  
pp. 5296-5305 ◽  
Author(s):  
Hee Taek Kim ◽  
Kei-Anne Baritugo ◽  
Young Hoon Oh ◽  
Sung Min Hyun ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
High Level ◽  
Level Production

Fermentative High-Level Production of 5-Hydroxyvaleric Acid by Metabolically Engineered Corynebacterium glutamicum

2021 ◽  
Vol 9 (6) ◽  
pp. 2523-2533
Author(s):  
Yu Jung Sohn ◽  
Minsoo Kang ◽  
Kei-Anne Baritugo ◽  
Jina Son ◽  
Kyoung Hee Kang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Hydroxyvaleric Acid ◽  
High Level ◽  
Level Production

Combining Protein and Metabolic Engineering Strategies for High-Level Production of O-Acetylhomoserine in Escherichia coli

2019 ◽  
Vol 8 (5) ◽  
pp. 1153-1167 ◽  
Author(s):  
Liang Wei ◽  
Qian Wang ◽  
Ning Xu ◽  
Jian Cheng ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
High Level ◽  
Level Production

High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

2015 ◽  
Vol 216 ◽  
pp. 142-148 ◽  
Author(s):  
Pablo Ravasi ◽  
Mauricio Braia ◽  
Florencia Eberhardt ◽  
Claudia Elena ◽  
Sebastián Cerminati ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C ◽  
Corynebacterium Glutamicum ◽  
High Level ◽  
Level Production

scholarly journals Multistep metabolic engineering of Bacillus licheniformis to improve pulcherriminic acid production

2019 ◽  
Author(s):  
Shiyi Wang ◽  
Huan Wang ◽  
Dan Zhang ◽  
Xiaoyun Li ◽  
Jin’ge Zhang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Bacillus Licheniformis ◽  
Acid Production ◽  
Iron Chelation ◽  
Pathogenic Fungi ◽  
Antibacterial Activities ◽  
Acid Yield ◽  
Cell Factories ◽  
High Level ◽  
Level Production

AbstractPulcherriminic acid, a cyclodipeptide, possesses the excellent antibacterial activities by chelating iron ions from environment, however, the low yield has hindered its application. In this study, high-level production of pulcherriminic acid was achieved by the multistep metabolic engineering of Bacillus licheniformis DWc9n*. Firstly, leucine (Leu) supply was increased by overexpressing the genes ilvBHC-leuABCD and ilvD involved in the Leu synthetic pathway and deleting the gene bkdAB encoding a branched-chain α-keto acid dehydrogenase. The intracellular Leu content and pulcherriminic acid yield of strain W2 reached 147.4 mg/g DCW and 189.9 mg/L respectively, which corresponded to a 226.7% and 49.1% higher than those of DWc9n*. Secondly, the strain W3 was obtained by overexpressing leucyl-tRNA synthase LeuS in W2, which lead to a 190% improvement compared to DWc9n*, pulcherriminic acid yield increase to 367.7 mg/L. Thirdly, overexpression of the cytochrome synthase gene cluster yvmC-cypX further increased the yield of pulcherriminic acid to 507.4 mg/L. Finally, the secretion capability was improved by overexpressing the pulcherriminic acid transporter gene yvmA. This resulted in the 556.1 mg/L pulcherriminic acid was produced in W4/pHY-yvmA, increased by 340% as compared with DWc9n*, and it is the highest pulcherriminic acid production currently reported. Taken together, this study provided an efficient strategy for enhancing pulcherriminic acid production and might also be applied for high-level production of other cyclodipeptides.ImportancePulcherriminic acid is a cyclodipeptide that derived from cyclo(L-Leu-L-Leu), which has the same iron chelation group with hydroxamate siderophores. Pulcherriminic acid producing strains can inhibit the growths of various bacteria and plant pathogenic fungi. However, the expected pulcherriminic acid yield was not achieved. This study reports efficient microbial production of pulcherriminic acid in Bacillus licheniformis DWc9n* via multistep metabolic engineering strategies. The combination of these interventions resulted in the establishment of a pulcherriminic acid overproducing strain. In combination with bioprocess engineering efforts, pulcherriminic acid was produced at a final yield of 556 mg/L in a shake flask. This is the highest pulcherriminic acid yield ever reported so far using rationally engineered microbial cell factories.

High-Level Production of the Natural Blue Pigment Indigoidine from Metabolically Engineered Corynebacterium glutamicum for Sustainable Fabric Dyes

2021 ◽  
Author(s):  
Mohammad Rifqi Ghiffary ◽  
Cindy Pricilia Surya Prabowo ◽  
Komal Sharma ◽  
Yuchun Yan ◽  
Sang Yup Lee ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Blue Pigment ◽  
High Level ◽  
Level Production

High-level production of l-homoserine using a non-induced, non-auxotrophic Escherichia coli chassis through metabolic engineering

2021 ◽  
Vol 327 ◽  
pp. 124814
Author(s):  
Yu Zhang ◽  
Minhua Wei ◽  
Guihong Zhao ◽  
Wenjie Zhang ◽  
Yingzi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
High Level ◽  
Level Production

High-Level Production of Tyrosol with Noninduced Recombinant Escherichia coli by Metabolic Engineering

2020 ◽  
Vol 68 (16) ◽  
pp. 4616-4623
Author(s):  
Wei Xu ◽  
Cui Yang ◽  
Yuanyuan Xia ◽  
Lihua Zhang ◽  
Chunxiao Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Recombinant Escherichia Coli ◽  
High Level ◽  
Level Production
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