Comparative 13 C‐metabolic flux analysis indicate elevation of ATP regeneration, carbon dioxide, and heat production in industrial Saccharomyces cerevisiae strains

2021 ◽  
pp. 2000438
Author(s):  
Futa Yatabe ◽  
Nobuyuki Okahashi ◽  
Taisuke Seike ◽  
Fumio Matsuda
Keyword(s):  
Carbon Dioxide ◽  
Saccharomyces Cerevisiae ◽  
Heat Production ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Atp Regeneration

scholarly journals Molecular Basis for Anaerobic Growth of Saccharomyces cerevisiae on Xylose, Investigated by Global Gene Expression and Metabolic Flux Analysis

2004 ◽  
Vol 70 (4) ◽  
pp. 2307-2317 ◽  
Author(s):  
Marco Sonderegger ◽  
Marie Jeppsson ◽  
Bärbel Hahn-Hägerdal ◽  
Uwe Sauer
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Global Gene Expression ◽  
Anaerobic Growth ◽  
Flux Analysis ◽  
Atp Production ◽  
Redox Imbalance ◽  
Chemostat Cultures

ABSTRACT Yeast xylose metabolism is generally considered to be restricted to respirative conditions because the two-step oxidoreductase reactions from xylose to xylulose impose an anaerobic redox imbalance. We have recently developed, however, a Saccharomyces cerevisiae strain that is at present the only known yeast capable of anaerobic growth on xylose alone. Using transcriptome analysis of aerobic chemostat cultures grown on xylose-glucose mixtures and xylose alone, as well as a combination of global gene expression and metabolic flux analysis of anaerobic chemostat cultures grown on xylose-glucose mixtures, we identified the distinguishing characteristics of this unique phenotype. First, the transcript levels and metabolic fluxes throughout central carbon metabolism were significantly higher than those in the parent strain, and they were most pronounced in the xylose-specific, pentose phosphate, and glycerol pathways. Second, differential expression of many genes involved in redox metabolism indicates that increased cytosolic NADPH formation and NADH consumption enable a higher flux through the two-step oxidoreductase reaction of xylose to xylulose in the mutant. Redox balancing is apparently still a problem in this strain, since anaerobic growth on xylose could be improved further by providing acetoin as an external NADH sink. This improved growth was accompanied by an increased ATP production rate and was not accompanied by higher rates of xylose uptake or cytosolic NADPH production. We concluded that anaerobic growth of the yeast on xylose is ultimately limited by the rate of ATP production and not by the redox balance per se, although the redox imbalance, in turn, limits ATP production.

Metabolic flux analysis of glucose/ethanol metabolism in Saccharomyces cerevisiae cultures

2018 ◽  
Vol 44 ◽  
pp. S115
Author(s):  
P. Comas Sanchez ◽  
I. Martinez Monge ◽  
M. Lecina ◽  
A. Casablancas ◽  
J.J. Cairó Badillo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Ethanol Metabolism ◽  
Flux Analysis

13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae

2015 ◽  
Vol 120 (5) ◽  
pp. 532-538 ◽  
Author(s):  
Kenshi Hayakawa ◽  
Shuichi Kajihata ◽  
Fumio Matsuda ◽  
Hiroshi Shimizu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis

scholarly journals Metabolic flux analysis of Saccharomyces cerevisiae in a sealed winemaking fermentation system

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Hua Li ◽  
Jing Su ◽  
Wen Ma ◽  
Anque Guo ◽  
Zuhua Shan ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Fermentation System

Theoretical aspects of 13C metabolic flux analysis with sole quantification of carbon dioxide labeling

2005 ◽  
Vol 29 (2) ◽  
pp. 121-133 ◽  
Author(s):  
Tae Hoon Yang ◽  
Elmar Heinzle ◽  
Christoph Wittmann
Keyword(s):  
Carbon Dioxide ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis

scholarly journals Metabolic Flux Analysis during the Exponential Growth Phase of Saccharomyces cerevisiae in Wine Fermentations

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71909 ◽  
Author(s):  
Manuel Quirós ◽  
Rubén Martínez-Moreno ◽  
Joan Albiol ◽  
Pilar Morales ◽  
Felícitas Vázquez-Lima ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Phase ◽  
Exponential Growth ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Exponential Growth Phase ◽  
Flux Analysis ◽  
Wine Fermentations

Metabolic flux analysis of Saccharomyces cerevisiae grown on glucose, glycerol or acetate by -labeling experiments

2003 ◽  
Vol 16 (3) ◽  
pp. 211-220 ◽  
Author(s):  
Huimin Zhang ◽  
Kazuyuki Shimizu ◽  
Shanjing Yao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis
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