scholarly journals Small angle X-ray scattering as a complementary tool for high-throughput structural studies

Biopolymers ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 517-530 ◽  
Author(s):  
Thomas D. Grant ◽  
Joseph R. Luft ◽  
Jennifer R. Wolfley ◽  
Hiro Tsuruta ◽  
Anne Martel ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
Structural Studies ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

scholarly journals Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

2011 ◽  
Vol 44 (5) ◽  
pp. 1090-1099 ◽  
Author(s):  
Josiane P. Lafleur ◽  
Detlef Snakenborg ◽  
Søren S. Nielsen ◽  
Magda Møller ◽  
Katrine N. Toft ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Small Angle ◽  
Structural Investigation ◽  
Adaptor Protein ◽  
Complete Analysis ◽  
Uv Absorbance ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

A new microfluidic sample-preparation system is presented for the structural investigation of proteins using small-angle X-ray scattering (SAXS) at synchrotrons. The system includes hardware and software features for precise fluidic control, sample mixing by diffusion, automated X-ray exposure control, UV absorbance measurements and automated data analysis. As little as 15 µl of sample is required to perform a complete analysis cycle, including sample mixing, SAXS measurement, continuous UV absorbance measurements, and cleaning of the channels and X-ray cell with buffer. The complete analysis cycle can be performed in less than 3 min. Bovine serum albumin was used as a model protein to characterize the mixing efficiency and sample consumption of the system. The N2 fragment of an adaptor protein (p120-RasGAP) was used to demonstrate how the device can be used to survey the structural space of a protein by screening a wide set of conditions using high-throughput techniques.

scholarly journals Small angle X-ray scattering as a high-throughput method to classify antimicrobial modes of action

2016 ◽  
Vol 1858 (5) ◽  
pp. 918-925 ◽  
Author(s):  
A.R. von Gundlach ◽  
V.M. Garamus ◽  
T. Gorniak ◽  
H.A. Davies ◽  
M. Reischl ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
Modes Of Action ◽  
X Ray Scattering ◽  
High Throughput Method ◽  
Ray Scattering

scholarly journals Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering

Methods ◽  
2011 ◽  
Vol 55 (4) ◽  
pp. 342-349 ◽  
Author(s):  
Jeremiah S. Joseph ◽  
Wei Liu ◽  
Joshua Kunken ◽  
Thomas M. Weiss ◽  
Hiro Tsuruta ◽  
...  
Keyword(s):  
Membrane Protein ◽  
High Throughput ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Ray Scattering

scholarly journals In situ capabilities of Small Angle X-ray Scattering

2019 ◽  
Vol 8 (1) ◽  
pp. 352-369 ◽  
Author(s):  
Jinghua Feng ◽  
Manfred Kriechbaum ◽  
Li (Emily) Liu
Keyword(s):  
Magnetic Field ◽  
High Throughput ◽  
Small Angle ◽  
Nanoscale Systems ◽  
X Ray ◽  
X Ray Scattering ◽  
Flow Through ◽  
Stretching Flow ◽  
Ray Scattering

Abstract Small Angle X-ray Scattering (SAXS) is an ideal characterization tool to explore nanoscale systems. In order to investigate nanostructural changes of materials under realistic sample environments, it is essential to equip SAXS with diverse in situ capabilities based on the corresponding requirements. In this paper, we highlight the representative experimental setups and corresponding applications of five widely used in situ capabilities: temperature, pressure, stretching, flow-through, and electric field. Additionally, we also briefly introduce other four in situ techniques including humidity, high-throughput, rheology, and magnetic field.

Small-angle X-ray scattering: a high-throughput technique for investigating archaeological bone preservation

2004 ◽  
Vol 31 (10) ◽  
pp. 1349-1359 ◽  
Author(s):  
J.C Hiller ◽  
M.J Collins ◽  
A.T Chamberlain ◽  
T.J Wess
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Bone Preservation ◽  
Ray Scattering
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