Identification and characterization of Cu2O- and ZnO-binding polypeptides byEscherichia coli cell surface display: toward an understanding of metal oxide binding

2004 ◽  
Vol 87 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Corrine K. Thai ◽  
Haixia Dai ◽  
M. S. R. Sastry ◽  
Mehmet Sarikaya ◽  
Daniel T. Schwartz ◽  
...  
Keyword(s):  
Cell Surface ◽  
Metal Oxide ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coli Cell ◽  
Identification And Characterization ◽  
Byescherichia Coli

scholarly journals Rapid characterization of spike variants via mammalian cell surface display

2021 ◽  
Vol 81 (24) ◽  
pp. 5099-5111.e8
Author(s):  
Kamyab Javanmardi ◽  
Chia-Wei Chou ◽  
Cynthia I. Terrace ◽  
Ankur Annapareddy ◽  
Tamer S. Kaoud ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mammalian Cell ◽  
Surface Display ◽  
Cell Surface Display ◽  
Rapid Characterization

Cell Surface Display and Characterization of Rhizopus oryzae Lipase in Pichia pastoris Using Sed1p as an Anchor Protein

2015 ◽  
Vol 71 (1) ◽  
pp. 150-155 ◽  
Author(s):  
Wenqian Li ◽  
Hao Shi ◽  
Huaihai Ding ◽  
Liangliang Wang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Cell Surface ◽  
Rhizopus Oryzae ◽  
Surface Display ◽  
Cell Surface Display ◽  
Rhizopus Oryzae Lipase ◽  
Anchor Protein

Physiological Improvement to Enhance Escherichia coli Cell-Surface Display via Reducing Extracytoplasmic Stress

2008 ◽  
Vol 24 (2) ◽  
pp. 293-301 ◽  
Author(s):  
N. Narayanan ◽  
C.P. Chou
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coli Cell

scholarly journals The cystine knot of a squash-type protease inhibitor as a structural scaffold for Escherichia coli cell surface display of conformationally constrained peptides

1999 ◽  
Vol 12 (9) ◽  
pp. 797-806 ◽  
Author(s):  
Andreas Christmann ◽  
Kerstin Walter ◽  
Alexander Wentzel ◽  
Ralph Krätzner ◽  
Harald Kolmar
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Protease Inhibitor ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coli Cell ◽  
Conformationally Constrained ◽  
Cystine Knot ◽  
Constrained Peptides

scholarly journals Evaluation of Staphylococcal Cell Surface Display and Flow Cytometry for Postselectional Characterization of Affinity Proteins in Combinatorial Protein Engineering Applications

2007 ◽  
Vol 73 (21) ◽  
pp. 6714-6721 ◽  
Author(s):  
John L�fblom ◽  
Julia Sandberg ◽  
Henrik Wern�rus ◽  
Stefan St�hl
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Dissociation Rate ◽  
Affibody Molecules ◽  
Combinatorial Protein Engineering ◽  
Protein Expression And Purification ◽  
Affinity Proteins

ABSTRACT For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directly on the cell surface. A model system comprising three closely related affibody molecules with different affinities for immunoglobulin G and an albumin binding domain with affinity for human serum albumin was used to investigate advantages and differences compared to biosensor technology in a side-by-side manner. Equilibrium dissociation constant (KD ) determinations as well as dissociation rate analysis were performed using both methods, and the results show that the on-cell determinations give both KD and dissociation rate values in a very fast and reproducible manner and that the relative affinities are very similar to the biosensor results. Interestingly, the results also show that there are differences between the absolute affinities determined with the two different technologies, and possible explanations for this are discussed. This work demonstrates the advantages of cell surface display for directed evolution of affinity proteins in terms of fast postselectional, on-cell characterization of candidate clones without the need for subcloning and subsequent protein expression and purification but also demonstrates that it is important to be aware that absolute affinities determined using different methods often vary substantially and that such comparisons therefore could be difficult.

Recent Advances in Cell Surface Display Technologies for Directed Protein Evolution

2021 ◽  
pp. 81-103
Author(s):  
Maryam Raeeszadeh‐Sarmazdeh ◽  
Wilfred Chen
Keyword(s):  
Cell Surface ◽  
Protein Evolution ◽  
Surface Display ◽  
Cell Surface Display ◽  
Recent Advances

Ligand fishing based on cell surface display of enzymes for inhibitor screening

2021 ◽  
pp. 338359
Author(s):  
Yun-Cong Yuan ◽  
Xiao-Lin Bai ◽  
Yi-Ming Liu ◽  
Xiao-Yue Tang ◽  
Hao Yuan ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Inhibitor Screening ◽  
Ligand Fishing

Cell Surface Display of the Chlamydial Glycolipid Exoantigen (GLXA) Demonstrated by Antibody-Dependent Complement-Mediated Cytotoxicity

2004 ◽  
Vol 49 (1) ◽  
Author(s):  
Wilmore C. Webley ◽  
Gary J. Vora ◽  
Elizabeth S. Stuart
Keyword(s):  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Glycolipid Exoantigen
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