Physiological Improvement to Enhance Escherichia coli Cell-Surface Display via Reducing Extracytoplasmic Stress

2008 ◽  
Vol 24 (2) ◽  
pp. 293-301 ◽  
Author(s):  
N. Narayanan ◽  
C.P. Chou
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coli Cell

scholarly journals The cystine knot of a squash-type protease inhibitor as a structural scaffold for Escherichia coli cell surface display of conformationally constrained peptides

1999 ◽  
Vol 12 (9) ◽  
pp. 797-806 ◽  
Author(s):  
Andreas Christmann ◽  
Kerstin Walter ◽  
Alexander Wentzel ◽  
Ralph Krätzner ◽  
Harald Kolmar
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Protease Inhibitor ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coli Cell ◽  
Conformationally Constrained ◽  
Cystine Knot ◽  
Constrained Peptides

scholarly journals Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA

AMB Express ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Meisam Jeiranikhameneh ◽  
Mohamad Reza Razavi ◽  
Shiva Irani ◽  
Seyed Davar Siadat ◽  
Mana Oloomi
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Classical Pathway

Epitope mapping and affinity purification of monospecific antibodies by Escherichia coli cell surface display of gene-derived random peptide libraries

2001 ◽  
Vol 257 (1-2) ◽  
pp. 163-173 ◽  
Author(s):  
Andreas Christmann ◽  
Alexander Wentzel ◽  
Christiane Meyer ◽  
Gregor Meyers ◽  
Harald Kolmar
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Epitope Mapping ◽  
Affinity Purification ◽  
Surface Display ◽  
Cell Surface Display ◽  
Peptide Libraries ◽  
Random Peptide ◽  
Random Peptide Libraries ◽  
Monospecific Antibodies

Cell Surface Display of Functional Macromolecule Fusions on Escherichia coli for Development of an Autofluorescent Whole-Cell Biocatalyst

2008 ◽  
Vol 42 (16) ◽  
pp. 6105-6110 ◽  
Author(s):  
Chao Yang ◽  
Qiao Zhao ◽  
Zheng Liu ◽  
Qiyun Li ◽  
Chuanling Qiao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Functional Cell Surface Display and Controlled Secretion of Diverse Agarolytic Enzymes by Escherichia coli with a Novel Ligation-Independent Cloning Vector Based on the Autotransporter YfaL

2012 ◽  
Vol 78 (9) ◽  
pp. 3051-3058 ◽  
Author(s):  
Hyeok-Jin Ko ◽  
Eunhye Park ◽  
Joseph Song ◽  
Taek Ho Yang ◽  
Hee Jong Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Fluorescent Protein ◽  
Surface Display ◽  
Cell Surface Display ◽  
Heterologous Proteins ◽  
Display System ◽  
Reporter Protein ◽  
Content Type ◽  
Ligation Independent Cloning

ABSTRACTAutotransporters have been employed as the anchoring scaffold for cell surface display by replacing their passenger domains with heterologous proteins to be displayed. We adopted an autotransporter (YfaL) ofEscherichia colifor the cell surface display system. The critical regions in YfaL for surface display were identified for the construction of a ligation-independent cloning (LIC)-based display system. The designed system showed no detrimental effect on either the growth of the host cell or overexpressing heterologous proteins on the cell surface. We functionally displayed monomeric red fluorescent protein (mRFP1) as a reporter protein and diverse agarolytic enzymes fromSaccharophagus degradans2-40, including Aga86C and Aga86E, which previously had failed to be functional expressed. The system could display different sizes of proteins ranging from 25.3 to 143 kDa. We also attempted controlled release of the displayed proteins by incorporating a tobacco etch virus protease cleavage site into the C termini of the displayed proteins. The maximum level of the displayed protein was 6.1 × 104molecules per a single cell, which corresponds to 5.6% of the entire cell surface of actively growingE. coli.

Anaplasma marginale major surface protein 1a directs cell surface display of tick BM95 immunogenic peptides on Escherichia coli

2008 ◽  
Vol 135 (4) ◽  
pp. 326-332 ◽  
Author(s):  
Mario Canales ◽  
Consuelo Almazán ◽  
José M. Pérez de la Lastra ◽  
José de la Fuente
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Cell Surface Display ◽  
Major Surface Protein ◽  
Immunogenic Peptides ◽  
Major Surface

Cell surface display of carbonic anhydrase on Escherichia coli using ice nucleation protein for CO2 sequestration

2011 ◽  
Vol 108 (12) ◽  
pp. 2853-2864 ◽  
Author(s):  
Li-Hai Fan ◽  
Ning Liu ◽  
Ming-Rui Yu ◽  
Shang-Tian Yang ◽  
Huan-Lin Chen
Keyword(s):  
Escherichia Coli ◽  
Carbonic Anhydrase ◽  
Cell Surface ◽  
Co2 Sequestration ◽  
Surface Display ◽  
Cell Surface Display ◽  
Ice Nucleation ◽  
Ice Nucleation Protein
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