A novel function for selenium in biological system: Selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production

2006 ◽  
Vol 95 (6) ◽  
pp. 1188-1197 ◽  
Author(s):  
Jinyou Zhang ◽  
David Robinson ◽  
Peter Salmon
Keyword(s):  
Monoclonal Antibody ◽  
Cell Growth ◽  
Chinese Hamster Ovary Cell ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Biological System ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Monoclonal Antibody Production ◽  
Highly Effective

scholarly journals 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250416
Author(s):  
Yuichi Aki ◽  
Yuta Katsumata ◽  
Hirofumi Kakihara ◽  
Koichi Nonaka ◽  
Kenshu Fujiwara
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Chemical Compounds ◽  
Ovary Cell ◽  
Monoclonal Antibody Production ◽  
Ovary Cells

There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to robustness, high productivity and ability to produce proteins with ideal glycans. Chemical compounds, such as dimethyl sulfoxide, lithium chloride, and butyric acid, have been shown to improve monoclonal antibody production in mammalian cell cultures. In this study, we aimed to discover new chemical compounds that can improve cell-specific antibody production in recombinant Chinese hamster ovary cells. Out of the 23,227 chemicals screened in this study, 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide was found to increase monoclonal antibody production. The compound suppressed cell growth and increased both cell-specific glucose uptake rate and the amount of intracellular adenosine triphosphate during monoclonal antibody production. In addition, the compound also suppressed the galactosylation on a monoclonal antibody, which is a critical quality attribute of therapeutic monoclonal antibodies. Therefore, the compound might also be used to control the level of the galactosylation for the N-linked glycans. Further, the structure-activity relationship study revealed that 2,5-dimethylpyrrole was the most effective partial structure of 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide on monoclonal antibody production. Further structural optimization of 2,5-dimethylpyrrole derivatives could lead to improved production and quality control of monoclonal antibodies.

Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture

1991 ◽  
Vol 34 (5) ◽  
pp. 559-564 ◽  
Author(s):  
P. M. Hayter ◽  
E. M. A. Curling ◽  
A. J. Baines ◽  
N. Jenkins ◽  
I. Salmon ◽  
...  
Keyword(s):  
Cell Growth ◽  
Batch Culture ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Interferon Production ◽  
Production Kinetics

The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Luis Toronjo‐Urquiza ◽  
Adelina E. Acosta‐Martin ◽  
David C. James ◽  
Tibor Nagy ◽  
Robert J. Falconer
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell

scholarly journals Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch cultures

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Huijie Niu ◽  
Jiaqi Wang ◽  
Mengjuan Liu ◽  
Miaomiao Chai ◽  
Liang Zhao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Fed Batch ◽  
Charge Heterogeneity ◽  
Batch Cultures

Optimization of chemically defined feed media for monoclonal antibody production in Chinese hamster ovary cells

2015 ◽  
Vol 120 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Shohei Kishishita ◽  
Satoshi Katayama ◽  
Kunihiko Kodaira ◽  
Yoshinori Takagi ◽  
Hiroki Matsuda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Monoclonal Antibody Production ◽  
Ovary Cells

Exploring the capabilities of fluorometric online monitoring on chinese hamster ovary cell cultivations producing a monoclonal antibody

2016 ◽  
Vol 32 (6) ◽  
pp. 1592-1600 ◽  
Author(s):  
Karen Schwab ◽  
Thomas Amann ◽  
Jakob Schmid ◽  
René Handrick ◽  
Friedemann Hesse
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Online Monitoring ◽  
Chinese Hamster ◽  
Ovary Cell
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