Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon-γ glycosylation during batch and fed-batch cultures of CHO cells

2008 ◽  
Vol 100 (4) ◽  
pp. 721-733 ◽  
Author(s):  
N. Kochanowski ◽  
F. Blanchard ◽  
R. Cacan ◽  
F. Chirat ◽  
E. Guedon ◽  
...  
Keyword(s):  
Cho Cells ◽  
Interferon Γ ◽  
Fed Batch ◽  
Nucleotide Sugar ◽  
Recombinant Interferon ◽  
Batch Cultures

RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells

2006 ◽  
Vol 8 (6) ◽  
pp. 509-522 ◽  
Author(s):  
Sing Fee Lim ◽  
Kok Hwee Chuan ◽  
Sen Liu ◽  
Sophia O.H. Loh ◽  
Beatrice Y.F. Chung ◽  
...  
Keyword(s):  
Cho Cells ◽  
Fed Batch ◽  
Batch Cultures

scholarly journals Lactate Control Enhances Growth Advantage in Fed-batch Cultures of Metabolically Engineered CHO Cells with Reduced Novel Growth-inhibitory Compound Formation

2021 ◽  
Author(s):  
Quentin Bethune ◽  
Cameron Harrington ◽  
Bhanu Mulukutla
Keyword(s):  
Amino Acid ◽  
Cho Cells ◽  
Fed Batch ◽  
Compound Formation ◽  
Knock Out ◽  
Batch Cultures ◽  
Growth Inhibitory ◽  
Inhibitory Compound ◽  
Cell Densities ◽  
Produce Amino Acid

CHO cells have been recently shown to produce amino acid catabolism derived byproducts, which accumulate in fed-batch cultures to growth-inhibitory levels. Residual amino acid limitation or genetic engineering strategies have been successfully employed to suppress production of these novel growth inhibitory metabolic byproducts. However, the growth advantage attained due to suppression of these metabolic byproducts in fed-batch cultures is more pronounced when lactate accumulation is also controlled. BCAT1 knock-out (KO) CHO cells, which produce negligible levels of the metabolic byproducts isovalerate, isobutyrate and 2-methylbutyrate, grow to significantly higher peak cell densities in fed-batch cultures with lactate control (HiPDOG) as compared to cultures without lactate control. Henceforth, strategies involving novel metabolic byproduct control should preferably include lactate control to more easily assess the enhanced cell growth and productivities attainable.

Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures

2007 ◽  
Vol 9 (5-6) ◽  
pp. 406-418 ◽  
Author(s):  
Chee Yong Yun ◽  
Sen Liu ◽  
Sing Fee Lim ◽  
Tianhua Wang ◽  
Beatrice Y.F. Chung ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cho Cells ◽  
Caspase 8 ◽  
Specific Inhibition ◽  
Fed Batch ◽  
Batch Cultures

Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures of CHO cells co‐fed with glucose and lactic acid

2021 ◽  
Author(s):  
Johanna Vappiani ◽  
Tom Eyster ◽  
Keegan Orzechowski ◽  
Diana Ritz ◽  
Pramthesh Patel ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cho Cells ◽  
Fed Batch ◽  
Batch Cultures

scholarly journals Metabolic engineering of CHO cells to alter lactate metabolism during fed-batch cultures

2016 ◽  
Vol 217 ◽  
pp. 122-131 ◽  
Author(s):  
Cécile Toussaint ◽  
Olivier Henry ◽  
Yves Durocher
Keyword(s):  
Metabolic Engineering ◽  
Cho Cells ◽  
Lactate Metabolism ◽  
Fed Batch ◽  
Batch Cultures

scholarly journals Production of Butyrate and Branched Chain Amino Acid Catabolic Byproducts by CHO Cells in Fed-batch Culture Enhances their Specific Productivity

2021 ◽  
Author(s):  
Cameron Harrington ◽  
Taylor Kalomeris ◽  
Michaela Jacobs ◽  
Gregory Hiller ◽  
Bhanu Chandra Mulukutla
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Cho Cells ◽  
Lactate Production ◽  
Specific Productivity ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures ◽  
Branched Chain Amino Acid ◽  
Branched Chain

Chinese hamster ovary (CHO) cells in fed-batch cultures produce several metabolic byproducts derived from amino acid catabolism, some of which accumulate to growth inhibitory levels. Controlling the accumulation of these byproducts has been shown to significantly enhance cell proliferation. Interestingly, some of these byproducts have physiological roles that go beyond inhibition of cell proliferation. In this study, we show that, in CHO cell fed-batch cultures, branched chain amino acid (BCAA) catabolism contributes to the formation of butyrate, a novel byproduct that is also a well-established specific productivity enhancer. Further, the other byproducts of BCAA catabolism, isovalerate and isobutyrate, which accumulate in CHO cell fed-batch cultures also enhance specific productivity. Additionally, the rate of production of these BCAA catabolic byproducts was negatively correlated with glucose uptake and lactate production rates. Limiting glucose supply to suppress glucose uptake and lactate production, like in case of fed-batch cultures employing HiPDOG technology, significantly enhances BCAA catabolic byproduct accumulation resulting in higher specific productivities.

scholarly journals Caspase activation, sialidase release and changes in sialylation pattern of recombinant human erythropoietin produced by CHO cells in batch and fed-batch cultures

2006 ◽  
Vol 51 (2) ◽  
pp. 67-79 ◽  
Author(s):  
Kok Hwee Chuan ◽  
Sing Fee Lim ◽  
Laurent Martin ◽  
Chee Yong Yun ◽  
Sophia O. H. Loh ◽  
...  
Keyword(s):  
Cho Cells ◽  
Recombinant Human Erythropoietin ◽  
Caspase Activation ◽  
Fed Batch ◽  
Batch Cultures ◽  
Human Erythropoietin

Modeling and parameter identification of microbial bioconversion in fed-batch cultures

2008 ◽  
Vol 18 (5) ◽  
pp. 458-464 ◽  
Author(s):  
Gang Wang ◽  
Enmin Feng ◽  
Zhilong Xiu
Keyword(s):  
Parameter Identification ◽  
Fed Batch ◽  
Batch Cultures
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