Mathematical modeling of the effects of ammonium ion on the intracellular pH of hybridoma cells

The description of the process and treatment facilities GUP "LenoblmolokoTikhvin". A mathematical model of the convective-diffusive transport and transformation of pollutants for the river Tikhvinka in the area of wastewater sue "LenoblmolokoTikhvin". The obtained two-dimensional stationary equation of convective-diffusion transfer of a nonconservative impurity is solved by the finite difference method. According to the developed model, the calculation of the anthropogenic load exerted by the water channel on the Tikhvinkariver was carried out with three variants of the design of the scattering water outlet for the following pollutants: ammonium-ion, nitrate-ion, nitrite-ion and phosphorus total. The maximum concentration of pollutants in the control range and the degree of mixing of pollutants were chosen as criteria for choosing the optimal design. According to the results of mathematical modeling it is shown that the optimal design of the scattering outlet consists of 7 scattering nozzles with a diameter of 200 mm, providing a reduction of the maximum concentrations in the control section by 12% compared to the scattering outlet with 4 drainage pipes.

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Model of Hydraulic Resistance When Forecasting Reverse Osmosis in Water Treatment

Membranes ◽

10.3390/membranes11050314 ◽

2021 ◽

Vol 11 (5) ◽

pp. 314

Author(s):

Janina Piekutin ◽

Urszula Kotowska

Keyword(s):

Mathematical Modeling ◽

Water Treatment ◽

Hydraulic Resistance ◽

Reverse Osmosis ◽

Hydraulic Model ◽

Treated Wastewater ◽

Ammonium Ion ◽

Pressure Filtration ◽

Filtration Resistance ◽

Membrane Change

The article presents research on the treatment of infiltration water with increased ammonium ion and nitrate(V) content through reverse osmosis. Then, research was conducted on the phenomena related to the decrease in the permeability of the membrane used for the research. The search for an appropriate interpretation of the phenomena was carried out using mathematical modeling. Based on the assumptions of the hydraulic model of the filtration resistance, calculations were made to forecast the efficiency of the osmotic membrane used in the discussed process. For this purpose, the following indicators were determined experimentally for the membrane: change in the volumetric flow of treated wastewater during low-pressure filtration, total hydraulic resistance, and component resistances, i.e., the resistance of the “new” membrane and resistances resulting from the reversible and irreversible fouling phenomena. It has been observed that irreversible resistance arises in the short and early stages of the process. The efficiency is determined by reversible resistance, which is confirmed by the literature.

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Effects of ammonium ion removal on growth and MAb production of hybridoma cells

Cytotechnology ◽

10.1007/bf00744318 ◽

1995 ◽

Vol 18 (1-2) ◽

pp. 35-50 ◽

Cited By ~ 4

Author(s):

Masatoshi Matsumura ◽

Fidel Rey P. Nayve

Keyword(s):

Ammonium Ion ◽

Hybridoma Cells ◽

Ion Removal ◽

Ammonium Ion Removal ◽

Mab Production

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Effects of ammonium ion removal on growth and MAb production of hybridoma cells

Animal Cell Technology: Developments Towards the 21st Century ◽

10.1007/978-94-011-0437-1_40 ◽

1995 ◽

pp. 247-262

Author(s):

Masatoshi Matsumura ◽

Fidel Rey P. Nayve

Keyword(s):

Ammonium Ion ◽

Hybridoma Cells ◽

Ion Removal ◽

Ammonium Ion Removal ◽

Mab Production

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Intracellular alkalinization potentiates slow inward current and prolonged bursting in a molluscan neuron

Journal of Neurophysiology ◽

10.1152/jn.1983.49.2.509 ◽

1983 ◽

Vol 49 (2) ◽

pp. 509-515 ◽

Cited By ~ 16

Author(s):

R. Gillette

Keyword(s):

Intracellular Ph ◽

Ammonium Ion ◽

Negative Slope ◽

Slow Inward Current ◽

Equilibrium Potential ◽

Buffer Solution ◽

Buccal Ganglion ◽

Ph Change ◽

Inward Current ◽

Intracellular Alkalinization

1. The bilaterally paired ventral white cells (VWCs) of the buccal ganglion of Pleurobranchaea drive the cyclic motor output of ingestive feeding behavior during prolonged and endogenously sustained burst episodes (7). The capacity to support burst episodes is specifically induced by appetitive (food) stimulation of chemosensory pathways (5). Cyclic 3',5'-adenosine monophosphate (cAMP) and its agonists also induce prolonged burst episodes (8) through potentiation of a slow inward current (6). 2. Intracellular alkalinization of the VWC by externally applied ammonium ion and methylamine (5-20 mM) induces bursting and enhances slow inward current measured under voltage-clamp conditions. The enhancement of slow inward current is seen in the induction or augmentation of a negative slope resistance region in the current-voltage relation and in the enhancement of slowly decaying inward current tails recorded near the K+ equilibrium potential following depolarizing voltage commands. 3. Intracellular injection of alkalinizing agents, bicarbonate ion and a strong buffer solution at pH 8.1, also enhance the inward current. In ammonium saline, enhancement of inward current is dependent on NH3 content, not NH4+; NH3 is the intracellular alkalinizing agent of ammonium saline. Therefore, the change in slow inward current is an effect specific to intracellular pH. 4. The time courses of inward current enhancement and intracellular pH change in NH4+ saline are similar. The results of this study suggest that normal fluctuations in intracellular pH may be significant determinants of the excitability and consequent activity of these and perhaps other neurons. The potential interaction of intracellular pH and cyclic AMP metabolism is discussed.

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