Foreign gene expression (?-galactosidase) during the cell cycle phases in recombinant CHO cells

1993 ◽  
Vol 42 (9) ◽  
pp. 1113-1123 ◽  
Author(s):  
Man Bock Gu ◽  
Paul Todd ◽  
Dhinakar S. Kompala
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cho Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Recombinant Cho Cells ◽  
Cell Cycle Phases

High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

1999 ◽  
Vol 10 (10) ◽  
pp. 1735-1737 ◽  
Author(s):  
Guofeng Zhang ◽  
Vladimir Budker ◽  
Jon A. Wolff
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tail Vein ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Continued initiation of DNA synthesis in arginine-deprived Chinese hamster ovary cells.

1977 ◽  
Vol 73 (1) ◽  
pp. 200-205 ◽  
Author(s):  
A S Weissfeld ◽  
H Rouse
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Multiplication ◽  
Ovary Cells ◽  
Cell Cycle Phases

When exponentially growing CHO cells were deprived of arginine (Arg), cell multiplication ceased after 12 h, but initiation of DNA synthesis continued: after 48 h of starvation with continuous [3H]thymidine exposure, 85% of the population had incorporated label, as detected autoradiographically. Consideration of the distribution of exponential cells in the various cell cycle phases leads to a calculation that most cells in G1 at the time that Arg was removed, as well as those in S, engaged in some DNA synthesis during starvation. In contrast, isoleucine (Ile)-starved cells did not initiate DNA synthesis, as has been reported by others. Experiments with cells synchronized by mitotic selection confirmed this difference in Arg- and Ile- deprived behavior, but also showed that cells which underwent the mitosis leads to G1 transition during Arg starvation remained arrested in G1 (G0?). The results suggest that Arg-deprived cells continue to maintain some proliferative function(s) while Ile-deprived cells do not.

scholarly journals Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors.

1990 ◽  
Vol 87 (1) ◽  
pp. 88-92 ◽  
Author(s):  
H. Daniell ◽  
J. Vivekananda ◽  
B. L. Nielsen ◽  
G. N. Ye ◽  
K. K. Tewari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tobacco Cells ◽  
Biolistic Delivery

CHLORAMPHENICOL INDUCIBILITY OF FOREIGN GENE EXPRESSION IN BACILLUS SUBTILIS

1984 ◽  
pp. 275-283 ◽  
Author(s):  
P.S. Lovett ◽  
D.M. Williams ◽  
E.J. Duvall ◽  
S. Mongkolsuk
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Foreign Gene ◽  
Foreign Gene Expression
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