Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector

1996 ◽  
Vol 59 (3) ◽  
pp. 349-360 ◽  
Author(s):  
Koji Sode ◽  
Naoaki Hatano ◽  
Masahiro Tatara
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Promoter Probe ◽  
Promoter Probe Vector

High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

1999 ◽  
Vol 10 (10) ◽  
pp. 1735-1737 ◽  
Author(s):  
Guofeng Zhang ◽  
Vladimir Budker ◽  
Jon A. Wolff
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tail Vein ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors.

1990 ◽  
Vol 87 (1) ◽  
pp. 88-92 ◽  
Author(s):  
H. Daniell ◽  
J. Vivekananda ◽  
B. L. Nielsen ◽  
G. N. Ye ◽  
K. K. Tewari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tobacco Cells ◽  
Biolistic Delivery

CHLORAMPHENICOL INDUCIBILITY OF FOREIGN GENE EXPRESSION IN BACILLUS SUBTILIS

1984 ◽  
pp. 275-283 ◽  
Author(s):  
P.S. Lovett ◽  
D.M. Williams ◽  
E.J. Duvall ◽  
S. Mongkolsuk
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Foreign Gene ◽  
Foreign Gene Expression

Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene‐expression differences betweenMut+andMutSstrains ofPichia pastoris

Yeast ◽  
2019 ◽  
Vol 36 (5) ◽  
pp. 285-296 ◽  
Author(s):  
Chrispian W. Theron ◽  
Julio Berrios ◽  
Sébastien Steels ◽  
Samuel Telek ◽  
Renaud Lecler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Green Fluorescent ◽  
Insight Into

scholarly journals Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide fromSorghum bicolorL. Moench

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Norazlina Ahmad ◽  
Rajnesh Sant ◽  
Milovan Bokan ◽  
Kathryn J. Steadman ◽  
Ian D. Godwin
Keyword(s):  
Gene Expression ◽  
Signal Peptide ◽  
Sweet Corn ◽  
Signal Sequence ◽  
Seed Storage ◽  
Foreign Gene ◽  
Regulatory Sequences ◽  
Fluorescent Properties ◽  
Gfp Expression ◽  
Foreign Gene Expression

Regulatory sequences with endosperm specificity are essential for foreign gene expression in the desired tissue for both grain quality improvement and molecular pharming. In this study, promoters of seed storage α-kafirin genes coupled with signal sequence (ss) were isolated fromSorghum bicolorL. Moench genomic DNA by PCR. The α-kafirin promoter (α-kaf) contains endosperm specificity-determining motifs, prolamin-box, the O2-box 1, CATC, and TATA boxes required for α-kafirin gene expression in sorghum seeds. The constructs pMB-Ubi-gfpand pMB-kaf-gfpwere microprojectile bombarded into various sorghum and sweet corn explants. GFP expression was detected on all explants using the Ubi promoter but only in seeds for the α-kaf promoter. This shows that the α-kaf promoter isolated was functional and demonstrated seed-specific GFP expression. The constructs pMB-Ubi-ss-gfpand pMB-kaf-ss-gfpwere also bombarded into the same explants. Detection of GFP expression showed that the signal peptide (SP)::GFP fusion can assemble and fold properly, preserving the fluorescent properties of GFP.

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