Enhancing cofactor recycling in the bioconversion of racemic alcohols to chiral amines with alcohol dehydrogenase and amine dehydrogenase by coupling cells and cell‐free system

2019 ◽  
Vol 116 (3) ◽  
pp. 536-542 ◽  
Author(s):  
Ji Liu ◽  
Zhi Li
Keyword(s):  
Alcohol Dehydrogenase ◽  
Chiral Amines ◽  
Free System ◽  
Cell Free System ◽  
Cofactor Recycling ◽  
Amine Dehydrogenase

scholarly journals Cell-free biosynthesis of ω-hydroxy acids boosted by a synergistic combination of alcohol dehydrogenases

2021 ◽  
Author(s):  
Susana Velasco ◽  
Javier Santiago-Arcos ◽  
Maria Grazia ◽  
Fernando López-Gallego
Keyword(s):  
Molecular Oxygen ◽  
Electron Acceptor ◽  
Enzyme System ◽  
Hydroxy Acids ◽  
Alcohol Dehydrogenases ◽  
Free System ◽  
Cell Free System ◽  
Whole Cells ◽  
Cofactor Recycling ◽  
Heterogeneous Biocatalysts

The activity orchestration of an unprecedented cell-free enzyme system with self-sufficient cofactor recycling enables the step-wise transformation of aliphatic diols into -hydroxy acids at the expense of molecular oxygen as electron acceptor. The efficiency of the biosynthetic route was maximized when two compatible alcohol dehydrogenases were selected as specialist biocatalysts for each one of the oxidative steps required for the oxidative lactonization of diols. The cell-free system reached up to 100% conversion using 100 mM of linear C5 diols, and performed the dessymetrization of prochiral branched diols into the corresponding -hydroxy acids with an exquisite enantioselectivity (ee > 99%). Green metrics demostrate a superior sustanability of this system compared to traditional metal catalysts and even to whole cells for the synthesis of 5-hydroxy petanoic acid. Finally, the cell-free system was assembled into a consortium of heterogeneous biocatalysts that allowed the enzyme reutilization. This cascade illustrates the potential of systems biocatalysis to access new heterofunctional molecules such as -hydroxy acids.

Isolation of globin mRNA from spleen of anemic rabbits and its translation in wheat embryo cell-free system

1978 ◽  
Vol 43 (4) ◽  
pp. 1184-1189
Author(s):  
Ota Fuchs ◽  
Jitka Borová ◽  
Přemysl Poňka ◽  
Jan Neuwirt
Keyword(s):  
Embryo Cell ◽  
Globin Mrna ◽  
Free System ◽  
Wheat Embryo ◽  
Cell Free System

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Synthesis of Ovalbumin in a Rabbit Reticulocyte Cell-free System Programmed with Hen Oviduct Ribonucleic Acid

1971 ◽  
Vol 246 (23) ◽  
pp. 7407-7410
Author(s):  
Robert E. Rhoads ◽  
G. Stanley McKnight ◽  
Robert T. Schimke
Keyword(s):  
Ribonucleic Acid ◽  
Free System ◽  
Cell Free System

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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