31P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics

R. Marshall Werner; Austin Johnson

doi:10.1002/bmb.21079

31P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics

Biochemistry and Molecular Biology Education ◽

10.1002/bmb.21079 ◽

2017 ◽

Vol 45 (6) ◽

pp. 509-514 ◽

Cited By ~ 1

Author(s):

R. Marshall Werner ◽

Austin Johnson

Keyword(s):

Enzyme Kinetics ◽

Pyruvate Kinase ◽

31P Nmr ◽

Kinase Reaction

Differentiation of nucleotide binding sites and role of metal ion in the adenylate kinase reaction by 31P NMR. Equilibria, interconversion rates, and NMR parameters of bound substrates

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)38123-1 ◽

1978 ◽

Vol 253 (4) ◽

pp. 1149-1158 ◽

Cited By ~ 3

Author(s):

B.D. Nageswara Rao ◽

M. Cohn ◽

L. Noda

Keyword(s):

Binding Sites ◽

Adenylate Kinase ◽

Metal Ion ◽

31P Nmr ◽

Nucleotide Binding ◽

Nucleotide Binding Sites ◽

Nmr Parameters ◽

Kinase Reaction

31P NMR studies of the arginine kinase reaction. Equilibrium constants and exchange rates at stoichiometric enzyme concentration.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)32930-7 ◽

1976 ◽

Vol 251 (22) ◽

pp. 6981-6986

Author(s):

B D Rao ◽

D H Buttlaire ◽

M Cohn

Keyword(s):

Exchange Rates ◽

31P Nmr ◽

Equilibrium Constants ◽

Arginine Kinase ◽

Enzyme Concentration ◽

Nmr Studies ◽

Kinase Reaction ◽

Reaction Equilibrium

Kinetic and Magnetic Resonance Studies of the Pyruvate Kinase Reaction

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)97641-6 ◽

1965 ◽

Vol 240 (1) ◽

pp. 238-246 ◽

Cited By ~ 21

Author(s):

Albert S. Mildvan ◽

Mildred Cohn

Keyword(s):

Magnetic Resonance ◽

Pyruvate Kinase ◽

Magnetic Resonance Studies ◽

Kinase Reaction

Free energy dissipation of the pyruvate kinase reaction has a minimum at cell metabolite concentrations

Biophysical Chemistry ◽

10.1016/0301-4622(83)80047-7 ◽

1983 ◽

Vol 18 (4) ◽

pp. 349-352

Author(s):

Mario Markus ◽

Theodor Plesser

Keyword(s):

Free Energy ◽

Energy Dissipation ◽

Pyruvate Kinase ◽

Kinase Reaction ◽

Metabolite Concentrations

Stereochemical studies of the pyruvate kinase reaction with (Z)- and (E)-phosphoenol-α-ketobutyrate

Biochemistry ◽

10.1021/bi00639a008 ◽

1977 ◽

Vol 16 (20) ◽

pp. 4382-4387 ◽

Cited By ~ 13

Author(s):

Mella Adlersberg ◽

Jean Dayan ◽

William E. Bondinell ◽

David B. Sprinson

Keyword(s):

Pyruvate Kinase ◽

Kinase Reaction

A rate-determining proton relay in the pyruvate kinase reaction

Biochemistry ◽

10.1021/bi00217a021 ◽

1991 ◽

Vol 30 (3) ◽

pp. 722-726 ◽

Cited By ~ 13

Author(s):

Irwin A. Rose ◽

Donald J. Kuo ◽

Jessie V. B. Warms

Keyword(s):

Pyruvate Kinase ◽

Kinase Reaction ◽

Proton Relay

A study of enzyme kinetics in perfused hearts from hypothyroid rats using 31P NMR☆A-M.L. Seymour, J.M. Keogh, G.K. Radda. Department of Biochemistry, Oxford University, South Parks Road, oxford OX1 3QU, U.K.

Journal of Molecular and Cellular Cardiology ◽

10.1016/0022-2828(83)90927-6 ◽

1983 ◽

Vol 15 ◽

pp. 52-52

Keyword(s):

Enzyme Kinetics ◽

31P Nmr ◽

Oxford University ◽

Perfused Hearts

Combination of 31P-NMR magnetization transfer and radioisotope exchange methods for assessment of an enzyme reaction mechanism: rate-determining steps of the creatine kinase reaction

Biochimica et Biophysica Acta (BBA) - Bioenergetics ◽

10.1016/0005-2728(90)90160-6 ◽

1990 ◽

Vol 1020 (3) ◽

pp. 290-304 ◽

Cited By ~ 15

Author(s):

V.V. Kupriyanov ◽

R.S. Balaban ◽

N.V. Lyulina ◽

A.Ya. Steinschneider ◽

V.A. Saks

Keyword(s):

Creatine Kinase ◽

Reaction Mechanism ◽

31P Nmr ◽

Magnetization Transfer ◽

Enzyme Reaction ◽

Kinase Reaction ◽

Creatine Kinase Reaction ◽

Rate Determining Steps

31P NMR studies of the thermodynamics and kinetics of the creatine kinase reaction

Magnetic Resonance in Medicine ◽

10.1002/mrm.1910110111 ◽

1989 ◽

Vol 11 (1) ◽

pp. 121-126 ◽

Cited By ~ 7

Author(s):

H. Eldar ◽

H. Degani

Keyword(s):

Creatine Kinase ◽

31P Nmr ◽

Nmr Studies ◽

Thermodynamics And Kinetics ◽

Kinase Reaction ◽

Creatine Kinase Reaction ◽

Kinetics Of

31P NMR studies of enzyme-bound substrates of rabbit muscle pyruvate kinase. Equilibrium constants, exchange rates, and NMR parameters.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)30127-8 ◽

1979 ◽

Vol 254 (8) ◽

pp. 2689-2696

Author(s):

B.D. Nageswara Rao ◽

F.J. Kayne ◽

M. Cohn

Keyword(s):

Exchange Rates ◽

Pyruvate Kinase ◽

31P Nmr ◽

Equilibrium Constants ◽

Rabbit Muscle ◽

Nmr Studies ◽

Nmr Parameters ◽

Muscle Pyruvate Kinase