Site-Specific Labelling of Native Mammalian Proteins for Single-Molecule FRET Measurements

ChemBioChem ◽  
2018 ◽  
Vol 19 (8) ◽  
pp. 780-783 ◽  
Author(s):  
Alexander Gust ◽  
Leonhard Jakob ◽  
Daniela M. Zeitler ◽  
Astrid Bruckmann ◽  
Kevin Kramm ◽  
...  
Keyword(s):  
Single Molecule ◽  
Site Specific ◽  
Single Molecule Fret ◽  
Mammalian Proteins ◽  
Specific Labelling

scholarly journals Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Louis Perdios ◽  
Alan R. Lowe ◽  
Giorgio Saladino ◽  
Tom D. Bunney ◽  
Nethaji Thiyagarajan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Single Molecule ◽  
Conformational Transition ◽  
Unnatural Amino Acid ◽  
Site Specific ◽  
Kinase Activation ◽  
Single Molecule Fret

scholarly journals A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

2017 ◽  
Vol 53 (57) ◽  
pp. 7986-7989 ◽  
Author(s):  
Fei Lou ◽  
Jie Yang ◽  
Si Wu ◽  
Sarah Perrett
Keyword(s):  
Single Molecule ◽  
Acceptor Pair ◽  
Site Specific ◽  
Single Molecule Fret ◽  
Donor Acceptor ◽  
Dimeric Protein ◽  
Donor Acceptor Pair ◽  
Single Molecule Analysis

Site-specific incorporation of donor–acceptor pair into a dimeric protein for single-molecule FRET analysis is realized based on a co-expression strategy.

scholarly journals Encoding Multiple Virtual Signals in DNA Barcodes with Single-Molecule FRET

Nano Letters ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1694-1701 ◽  
Author(s):  
Sung Hyun Kim ◽  
Hyunwoo Kim ◽  
Hawoong Jeong ◽  
Tae-Young Yoon
Keyword(s):  
Single Molecule ◽  
Dna Barcodes ◽  
Single Molecule Fret

Single-Molecule FRET-Based Dynamic DNA Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Anoja Megalathan ◽  
Kalani M. Wijesinghe ◽  
Soma Dhakal
Keyword(s):  
Single Molecule ◽  
Dna Sensor ◽  
Single Molecule Fret
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