scholarly journals Front Cover: Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction (ChemBioChem 3/2018)

ChemBioChem ◽  
2018 ◽  
Vol 19 (3) ◽  
pp. 196-196
Author(s):  
Aitao Li ◽  
Carlos G. Acevedo-Rocha ◽  
Zhoutong Sun ◽  
Tony Cox ◽  
Jia Lucy Xu ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Combinatorial Library ◽  
Solid Phase ◽  
Gene Synthesis ◽  
High Fidelity ◽  
Gene Assembly ◽  
Front Cover ◽  
Efficient Gene ◽  
On Chip ◽  
Evolution Of Proteins

Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?

ChemBioChem ◽  
2018 ◽  
Vol 19 (19) ◽  
pp. 2023-2032 ◽  
Author(s):  
Aitao Li ◽  
Zhoutong Sun ◽  
Manfred T. Reetz
Keyword(s):  
Directed Evolution ◽  
Solid Phase ◽  
Gene Synthesis ◽  
Mutant Library ◽  
Library Construction ◽  
The Future

Speeding up Directed Evolution: Combining the Advantages of Solid-Phase Combinatorial Gene Synthesis with Statistically Guided Reduction of Screening Effort

2014 ◽  
Vol 4 (3) ◽  
pp. 317-331 ◽  
Author(s):  
Sabrina Hoebenreich ◽  
Felipe E. Zilly ◽  
Carlos G. Acevedo-Rocha ◽  
Matías Zilly ◽  
Manfred T. Reetz
Keyword(s):  
Directed Evolution ◽  
Solid Phase ◽  
Gene Synthesis

scholarly journals Integrating Gene Synthesis and Microfluidic Protein Analysis for Rapid Protein Engineering

2015 ◽  
Author(s):  
Matthew C Blackburn ◽  
Ekaterina Petrova ◽  
Bruno E Correia ◽  
Sebastian Josef Maerkl
Keyword(s):  
Protein Design ◽  
Solid Phase ◽  
Synthesis Method ◽  
Gene Synthesis ◽  
Sequence Specificity ◽  
Quantitative Characterization ◽  
Synthetic Genes ◽  
In Silico Modeling ◽  
On Chip ◽  
Protein Biophysics

The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology, and synthetic biology. Synthetic genes are becoming a commodity, but integrated approaches have yet to be developed that take full advantage of gene synthesis. We developed a solid-phase gene synthesis method based on asymmetric primer extension (APE) and coupled this process directly to high-throughput, on-chip protein expression, purification, and characterization (mechanically induced trapping of molecular interactions, MITOMI). By completely circumventing molecular cloning and cell-based steps, APE-MITOMI reduces the time between protein design and quantitative characterization to 3-4 days. With APE- MITOMI we synthesized and characterized over 440 zinc-finger (ZF) transcription factors (TF), showing that although ZF TFs can be readily engineered to recognize a particular DNA sequence, engineering the precise binding energy landscape remains challenging. We also found that it is possible to engineer ZF – DNA affinity precisely and independently of sequence specificity and that in silico modeling can explain some of the observed affinity differences. APE-MITOMI is a generic approach that should facilitate fundamental studies in protein biophysics, and protein design/engineering.

scholarly journals Advances in the directed evolution of proteins

2014 ◽  
Vol 22 ◽  
pp. 129-136 ◽  
Author(s):  
Michael D Lane ◽  
Burckhard Seelig
Keyword(s):  
Directed Evolution ◽  
Evolution Of Proteins

Directed Evolution of Proteins for Device Applications

2008 ◽  
Author(s):  
Jeremy Koscielecki ◽  
Jason Hillebrecht ◽  
Robert Birge
Keyword(s):  
Directed Evolution ◽  
Device Applications ◽  
Evolution Of Proteins

Design Techniques for Microfluidic Devices Implementation Applicable to Chemical Analysis Systems

2019 ◽  
pp. 195-222 ◽  
Author(s):  
Reinaldo Lucas dos Santos Rosa ◽  
Antonio Carlos Seabra
Keyword(s):  
Chemical Analysis ◽  
Solid Phase ◽  
Microfluidic Devices ◽  
Water Quality Monitoring ◽  
Analysis Process ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
On Chip ◽  
Separation Cell ◽  
High Level

This chapter provides a guide for microfluidic devices development and optimization focused on chemical analysis applications, which includes medicine, biology, chemistry, and environmental monitoring, showing high-level performance associated with a specific functionality. Examples are chemical analysis, solid phase extraction, chromatography, immunoassay analysis, protein and DNA separation, cell sorting and manipulation, cellular biology, and mass spectrometry. In this chapter, most information is related to microfluidic devices design and fabrication used to perform several steps concerning chemical analysis, process preparation of reagents, samples reaction and detection, regarding water quality monitoring. These steps are especially relevant to lab-on-chip (LOC) and micro-total-analysis-systems (μTAS). μTAS devices are developed in order to simplify analytical chemist work, incorporating several analytical procedures into flow systems. In the case of miniaturized devices, the analysis time is reduced, and small volumes (nL) can be used.

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