scholarly journals Cover Feature: Characterization of a Tryptophan 6‐Halogenase from Streptomyces albus and Its Regioselectivity Determinants (ChemBioChem 10/2020)

ChemBioChem ◽  
2020 ◽  
Vol 21 (10) ◽  
pp. 1386-1386
Author(s):  
Jeongchan Lee ◽  
Joonwon Kim ◽  
Hyun Kim ◽  
Eun‐Jung Kim ◽  
Hee‐Jin Jeong ◽  
...  
Keyword(s):  
Streptomyces Albus ◽  
Feature Characterization

scholarly journals Cover Feature: Characterization of Phosphoranes Obtained by the Spontaneous Carbonyl‐Catalyzed Phosphorylation of Monosaccharides and Polyols in Aqueous Media (ChemBioChem 4/2020)

ChemBioChem ◽  
2020 ◽  
Vol 21 (4) ◽  
pp. 430-430
Author(s):  
Maria Perez‐Ramirez ◽  
Isaac Agyekum ◽  
Barnabas Otoo
Keyword(s):  
Aqueous Media ◽  
Feature Characterization

scholarly journals Characterization of Streptomyces albus 18-kilodalton heat shock-responsive protein.

1995 ◽  
Vol 177 (11) ◽  
pp. 2998-3003 ◽  
Author(s):  
P Servant ◽  
P Mazodier
Keyword(s):  
Heat Shock ◽  
Streptomyces Albus

scholarly journals Polarization imaging feature characterization of different endometrium phases by machine learning

OSA Continuum ◽  
2021 ◽  
Author(s):  
Yue Yao ◽  
Min Zuo ◽  
Yang Dong ◽  
Liyun Shi ◽  
Yuanhuan Zhu ◽  
...  
Keyword(s):  
Machine Learning ◽  
Imaging Feature ◽  
Polarization Imaging ◽  
Feature Characterization

scholarly journals Better prognostic determination and feature characterization of cutaneous melanoma through integrative genomic analysis

Aging ◽  
2019 ◽  
Vol 11 (14) ◽  
pp. 5081-5107 ◽  
Author(s):  
Xia Li ◽  
Yunpeng Cai
Keyword(s):  
Cutaneous Melanoma ◽  
Genomic Analysis ◽  
Feature Characterization

Feature characterization of scarring and non-scarring types of alopecia by multiphoton microscopy (Conference Presentation)

2018 ◽  
Author(s):  
Inga Saknite ◽  
Manuel Valdebran ◽  
Jessica Lin ◽  
Griffin R. Lentsch ◽  
Joshua N. Williams ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Feature Characterization

scholarly journals Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae

1998 ◽  
Vol 180 (6) ◽  
pp. 1381-1388 ◽  
Author(s):  
Rosario Muñoz ◽  
Rubens López ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence ◽  
Frameshift Mutation ◽  
Lactobacillus Helveticus ◽  
Inverted Repeats ◽  
Insertion Sequences ◽  
Amino Acid Residues ◽  
Streptomyces Albus

ABSTRACT We describe the characterization of a new insertion sequence, IS1515, identified in the genome of Streptococcus pneumoniae I41R, an unencapsulated mutant isolated many years ago (R. Austrian, H. P. Bernheimer, E. E. B. Smith, and G. T. Mills, J. Exp. Med. 110:585–602, 1959). A copy of this element located in the cap1E I41R gene was sequenced. The 871-bp-long IS1515 element possesses 12-bp perfect inverted repeats and generates a 3-bp target duplication upon insertion. The IS encodes a protein of 271 amino acid residues similar to the putative transposases of other insertion sequences, namely IS1381 from S. pneumoniae, ISL2from Lactobacillus helveticus, IS702 from the cyanobacterium Calothrix sp. strain PCC 7601, and IS112 from Streptomyces albus G. IS1515 appears to be present in the genome of most type 1 pneumococci in a maximum of 13 copies, although it has also been found in the chromosome of pneumococcal isolates belonging to other serotypes. We have found that the unencapsulated phenotype of strain I41R is the result of both the presence of an IS1515 copy and a frameshift mutation in the cap1E I41Rgene. Precise excision of the IS was observed in the type 1 encapsulated transformants isolated in experiments designed to repair the frameshift. These results reveal that IS1515 behaves quite differently from other previously described pneumococcal insertion sequences. Several copies of IS1515 were also able to excise and move to another locations in the chromosome ofS. pneumoniae. To our knowledge, this is the first report of a functional IS in pneumococcus.

scholarly journals Characterization of the groEL-like genes in Streptomyces albus.

1991 ◽  
Vol 173 (22) ◽  
pp. 7382-7386 ◽  
Author(s):  
P Mazodier ◽  
G Guglielmi ◽  
J Davies ◽  
C J Thompson
Keyword(s):  
Streptomyces Albus

scholarly journals Cloning and Characterization of the Polyether Salinomycin Biosynthesis Gene Cluster of Streptomyces albus XM211

2011 ◽  
Vol 78 (4) ◽  
pp. 994-1003 ◽  
Author(s):  
Chunyan Jiang ◽  
Hougen Wang ◽  
Qianjin Kang ◽  
Jing Liu ◽  
Linquan Bai
Keyword(s):  
Gene Cluster ◽  
Animal Husbandry ◽  
Oxidative Cyclization ◽  
Degenerate Primers ◽  
Biosynthesis Gene Cluster ◽  
Content Type ◽  
Feeding Experiments ◽  
Streptomyces Albus ◽  
Polyketide Chain

ABSTRACTSalinomycin is widely used in animal husbandry as a food additive due to its antibacterial and anticoccidial activities. However, its biosynthesis had only been studied by feeding experiments with isotope-labeled precursors. A strategy with degenerate primers based on the polyether-specific epoxidase sequences was successfully developed to clone the salinomycin gene cluster. Using this strategy, a putative epoxidase gene,slnC, was cloned from the salinomycin producerStreptomyces albusXM211. The targeted replacement ofslnCand subsequenttrans-complementation proved its involvement in salinomycin biosynthesis. A 127-kb DNA region containingslnCwas sequenced, including genes for polyketide assembly and release, oxidative cyclization, modification, export, and regulation. In order to gain insight into the salinomycin biosynthesis mechanism, 13 gene replacements and deletions were conducted. IncludingslnC, 7 genes were identified as essential for salinomycin biosynthesis and putatively responsible for polyketide chain release, oxidative cyclization, modification, and regulation. Moreover, 6 genes were found to be relevant to salinomycin biosynthesis and possibly involved in precursor supply, removal of aberrant extender units, and regulation. Sequence analysis and a series of gene replacements suggest a proposed pathway for the biosynthesis of salinomycin. The information presented here expands the understanding of polyether biosynthesis mechanisms and paves the way for targeted engineering of salinomycin activity and productivity.

Isolation, partial characterization of the cytoplasmic membrane fraction of Streptomyces albus G and dd-carboxypeptidase localization

1972 ◽  
Vol 81 (3) ◽  
pp. 273-288 ◽  
Author(s):  
Emilio Mu�oz ◽  
Alberto Marquet ◽  
Vicente Larraga ◽  
Jacques Coyette
Keyword(s):  
Membrane Fraction ◽  
Cytoplasmic Membrane ◽  
Partial Characterization ◽  
Streptomyces Albus
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