Cytoplasmic extension peptide of Pichia pastoris glucose sensor Gss1 is not compulsory for glucose signalling

2013 ◽  
Vol 38 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Andriy S. Polupanov ◽  
Andriy A. Sibirny
Keyword(s):  
Pichia Pastoris ◽  
Glucose Sensor ◽  
Cytoplasmic Extension ◽  
Glucose Signalling

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

1990 ◽  
Vol 48 (3) ◽  
pp. 860-861
Author(s):  
Lorna K. Mayo ◽  
Kenneth C. Moore ◽  
Mark A. Arnold
Keyword(s):  
Glucose Sensor ◽  
Low Voltage ◽  
Glucose Sensors ◽  
Polymeric Membranes ◽  
Artificial Endocrine Pancreas ◽  
Self Monitoring ◽  
Conducting Materials ◽  
Insulin Dependent ◽  
Living Tissues ◽  
Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

Abstract #32: A Prospective, 21-Day Trial of a Transcutaneous, Real-Time Continuous Glucose Sensor Demonstrates Improvement in Glycemic Excursions

2006 ◽  
Vol 12 ◽  
pp. 7
Author(s):  
Lois G. Jovanovic ◽  
Howard Zisser ◽  
Timothy Bailey ◽  
Roy Kaplan ◽  
Satish Garg
Keyword(s):  
Real Time ◽  
Glucose Sensor

A subcutaneously implanted glucose sensor: sensor signal and blood glucose following intravenous glucose load or insulin administration in normal and diabetic rats

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S181 ◽  
Author(s):  
M. DOLDERER ◽  
R. V. WARHADPANDE ◽  
C. ZAWADIL ◽  
H. ZIER ◽  
W. KERNER ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Sensor ◽  
Diabetic Rats ◽  
Insulin Administration ◽  
Glucose Load ◽  
Sensor Signal ◽  
Intravenous Glucose ◽  
Intravenous Glucose Load

Optimization of fermentation conditions for the production of recombinant hpdgf-bb in Pichia pastoris

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Duong Long Duy ◽  
Pham Minh Vu ◽  
Nguyen Tri Nhan ◽  
Tran Linh Thuoc ◽  
Dang Thi Phuong Thao
Keyword(s):  
Pichia Pastoris ◽  
Fermentation Conditions ◽  
Optimization Of Fermentation

965-P: Glucose Sensor Accuracy after Subcutaneous Glucagon Injections Near to Sensor Site

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 965-P
Author(s):  
CHRISTIAN LAUGESEN ◽  
SIGNE SCHMIDT ◽  
RIKKE TETZSCHNER ◽  
KIRSTEN NØRGAARD ◽  
AJENTHEN RANJAN
Keyword(s):  
Glucose Sensor ◽  
Sensor Accuracy

903-P: Hydroxyurea Resulting in False Elevation of Continuous Glucose Sensor Values

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 903-P
Author(s):  
SIOBHAN E. TELLEZ ◽  
LINDSEY HORNUNG ◽  
JOSHUA D. COURTER ◽  
MAISAM ABU-EL-HAIJA ◽  
JAIMIE D. NATHAN ◽  
...  
Keyword(s):  
Glucose Sensor

Application of chronic intravascular blood glucose sensor in dogs

Diabetes ◽  
1990 ◽  
Vol 39 (12) ◽  
pp. 1519-1526 ◽  
Author(s):  
J. C. Armour ◽  
J. Y. Lucisano ◽  
B. D. McKean ◽  
D. A. Gough
Keyword(s):  
Blood Glucose ◽  
Glucose Sensor

Banting Lecture 1995. A lesson in metabolic regulation inspired by the glucokinase glucose sensor paradigm

Diabetes ◽  
1996 ◽  
Vol 45 (2) ◽  
pp. 223-241 ◽  
Author(s):  
F. M. Matschinsky
Keyword(s):  
Metabolic Regulation ◽  
Glucose Sensor

Cloning and Expression of Recombinant Human Insulin Gene in Pichia pastoris

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Pichia Pastoris ◽  
Human Insulin ◽  
Expression Vector ◽  
Expression System ◽  
Insulin Gene ◽  
Cloning And Expression ◽  
Pcr Analysis ◽  
Major Band ◽  
Human Insulin Gene ◽  
Pichia Pastoris Expression System

The main goal of the current study was cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand, by using two sets of specific primers contain EcoR1 and Notl restriction sites. The amplified preproinsulin gene fragment was double digested with EcoRI and Not 1 restriction enzymes, then inserted into pPIC9K expression vector. The new pPIC9K-hpi constructive expression vector was transformed by the heat-shock method into the E.coli DH5α competent cells. pPic9k –hpi, which was propagated in the positive transformant E. coli cells, was isolated from cells and then linearised by restriction enzyme SalI, then transformed into Pichia pastoris GS115 using electroporation method. Genomic DNA of His+ transformants cell was extracted and used as a template for PCR analysis. The results showed, that the pPic9k – hpi was successfully integrated into the P. pastoris genome, for selected His+ transformants clones on the anticipated band at 330 bp, which is corresponded to the theoretical molecular size of the human insulin gene. To follow the insulin expression in transformans, Tricine–SDS gel electrophoresis and Western blot analysis were conducted. The results showed a successful expression of recombinant protein was detected by the presence of a single major band with about (5.8 KDa) on the gel. These bands correspond well with the size of human insulin with the theoretical molecular weight (5.8 KDa).

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