Porcine spermatogonial stem cells self-renew effectively in a three dimensional culture microenvironment

2017 ◽  
Vol 41 (12) ◽  
pp. 1316-1324 ◽  
Author(s):  
Ji Eun Park ◽  
Min Hee Park ◽  
Min Seong Kim ◽  
Yeo Reum Park ◽  
Jung Im Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
Three Dimensional Culture

scholarly journals A Review on Application of Three Dimensional Culture and Testicular Scaffolds to Induction of in-Vitro Spermatogenesis

2020 ◽  
Author(s):  
Nasrin Majidi Gharenaz ◽  
Mansoureh Movahedin ◽  
Samiyeh Majidi ◽  
Zohreh Mazaheri
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Spermatogonial Stem Cells ◽  
Ethical Challenges ◽  
Agar Culture ◽  
In Vitro Spermatogenesis ◽  
Review Study ◽  
Three Dimensional Culture

Introduction: Induction of in vitro spermatogenesis can be useful for infertility treatment in azoospermic patients and those undergoing chemotherapy. Different culture systems have been used to achieve this goal. This review study was performed with the aim to evaluate the application of 3D culture and testicular scaffolds in the establishment of in vitro spermatogenesis. In this review study, the information on the application of 3D culture and testicular scaffolds in induction of in vitro spermatogenesis was searched in databases such as SID, Magiran, PubMed, Irandoc, Iranmedx Scopus, Google Scholar, Web of Science using the keywords of three dimensional culture, testicular scaffold, spermatogenesis, spermatogonial stem cells without time limitation. Data analysis was carried out qualitatively. Finally, 35 papers in English and Persian were used to compile the article. In order to induce of in vitro spermatogenesis, three-dimensional culture methods such as testicular tissue culture, soft agar culture system, natural biomaterial scaffolds such as collagen, and scaffolds derived from decellularized testis have been used. Conclusion: Three-dimensional culture using spermatogonial stem cells and scaffolds can be used in vitro for induction of spermatogenesis, but there are further technical and ethical challenges in the path of fertile sperm production for the treatment of infertility.  

scholarly journals Differentiation of spermatogonial stem cells by soft agar three-dimensional culture system

2019 ◽  
Vol 47 (1) ◽  
pp. 1772-1781 ◽  
Author(s):  
Elham Mohammadzadeh ◽  
Tooba Mirzapour ◽  
Mohammad Reza Nowroozi ◽  
Hamid Nazarian ◽  
Abbas Piryaei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Culture System ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
Soft Agar ◽  
Three Dimensional Culture

Evaluation of alginate hydrogel cytotoxicity on three-dimensional culture of type A spermatogonial stem cells

2017 ◽  
Vol 95 ◽  
pp. 888-894 ◽  
Author(s):  
Maryam Jalayeri ◽  
Afshin Pirnia ◽  
Elaheh Poorazizi Najafabad ◽  
Ali Mohammad Varzi ◽  
Mohammadreza Gholami
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Type A ◽  
Spermatogonial Stem Cells ◽  
Alginate Hydrogel ◽  
Three Dimensional Culture

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

scholarly journals Substrate-independent immunomodulatory characteristics of mesenchymal stem cells in three-dimensional culture

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206811 ◽  
Author(s):  
Jing Li ◽  
Tong Chen ◽  
Xiahe Huang ◽  
Yunshan Zhao ◽  
Bin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Three Dimensional Culture ◽  
Substrate Independent
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