scholarly journals A Comparison of Three-Dimensional Culture Systems to Evaluate In Vitro Chondrogenesis of Equine Bone Marrow-Derived Mesenchymal Stem Cells

2013 ◽  
Vol 19 (19-20) ◽  
pp. 2275-2283 ◽  
Author(s):  
Ashlee E. Watts ◽  
Jeremy C. Ackerman-Yost ◽  
Alan J. Nixon
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Culture Systems ◽  
Three Dimensional Culture ◽  
Equine Bone

scholarly journals The Role of Glucose, Serum, and Three-Dimensional Cell Culture on the Metabolism of Bone Marrow-Derived Mesenchymal Stem Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Byron Deorosan ◽  
Eric A. Nauman
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Glucose Concentration ◽  
Metabolic Response ◽  
Three Dimensional ◽  
Metabolic State ◽  
Three Dimensional Culture

Mesenchymal stem cells (MSCs) have become a critical addition to all facets of tissue engineering. While mostin vitroresearch has focused on their behavior in two-dimensional culture, relatively little is known about the cells' behavior in three-dimensional culture, especially with regard to their metabolic state. To evaluate MSC metabolism during twodimensional culture, murine bone marrow-derived MSCs were cultured for one week using twelve different medium compositions, varying in both glucose and fetal bovine serum (FBS) concentrations. The results indicate that glucose concentration was the more important factor in sustaining cell growth and viability. To evaluate metabolic state during three-dimensional culture, MSCs were cultured for one week using two different medium compositions and two different concentrations of collagen gel matrix. The medium compositions only varied in glucose concentration. The results indicate that glucose and extracellular matrix were significant factors in the metabolic response of the cells. However, cells cultured in low density collagen exhibited considerable cell death, likely because of physical contraction of the collagen hydrogel which was not observed in the higher density collagen. These findings will be useful to the development ofin vitrocell culture models that properly mimicin vivophysiological processes.

Effects of continuous passage on the immunomodulatory properties of equine bone marrow-derived mesenchymal stem cells in vitro

2021 ◽  
Vol 234 ◽  
pp. 110203
Author(s):  
Shannon S. Connard ◽  
Renata L. Linardi ◽  
Kayla M. Even ◽  
Alix K. Berglund ◽  
Lauren V. Schnabel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Immunomodulatory Properties ◽  
Equine Bone

Can Extracorporeal Shockwave Promote Osteogenesis of Equine Bone Marrow-Derived Mesenchymal Stem Cells In Vitro?

2020 ◽  
Vol 29 (2) ◽  
pp. 110-118
Author(s):  
Aimee C. Colbath ◽  
John D. Kisiday ◽  
Jennifer N. Phillips ◽  
Laurie R. Goodrich
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Extracorporeal Shockwave ◽  
Equine Bone

Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems

2015 ◽  
Vol 53 (3) ◽  
pp. 1862-1872 ◽  
Author(s):  
Faezeh Faghihi ◽  
Esmaeil Mirzaei ◽  
Jafar Ai ◽  
Abolfazl Lotfi ◽  
Forough Azam Sayahpour ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Three Dimensional ◽  
Differentiation Potential ◽  
Culture Systems ◽  
Three Dimensional Culture

Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells

2013 ◽  
Vol 95 (2) ◽  
pp. 693-698 ◽  
Author(s):  
Jong-pil Seo ◽  
Nao Tsuzuki ◽  
Shingo Haneda ◽  
Kazutaka Yamada ◽  
Hidefumi Furuoka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
In Vitro Expansion ◽  
Fetal Bovine ◽  
Equine Bone
Sign in / Sign up

Export Citation Format

Share Document