scholarly journals A Review on Application of Three Dimensional Culture and Testicular Scaffolds to Induction of in-Vitro Spermatogenesis

2020 ◽  
Author(s):  
Nasrin Majidi Gharenaz ◽  
Mansoureh Movahedin ◽  
Samiyeh Majidi ◽  
Zohreh Mazaheri
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Spermatogonial Stem Cells ◽  
Ethical Challenges ◽  
Agar Culture ◽  
In Vitro Spermatogenesis ◽  
Review Study ◽  
Three Dimensional Culture

Introduction: Induction of in vitro spermatogenesis can be useful for infertility treatment in azoospermic patients and those undergoing chemotherapy. Different culture systems have been used to achieve this goal. This review study was performed with the aim to evaluate the application of 3D culture and testicular scaffolds in the establishment of in vitro spermatogenesis. In this review study, the information on the application of 3D culture and testicular scaffolds in induction of in vitro spermatogenesis was searched in databases such as SID, Magiran, PubMed, Irandoc, Iranmedx Scopus, Google Scholar, Web of Science using the keywords of three dimensional culture, testicular scaffold, spermatogenesis, spermatogonial stem cells without time limitation. Data analysis was carried out qualitatively. Finally, 35 papers in English and Persian were used to compile the article. In order to induce of in vitro spermatogenesis, three-dimensional culture methods such as testicular tissue culture, soft agar culture system, natural biomaterial scaffolds such as collagen, and scaffolds derived from decellularized testis have been used. Conclusion: Three-dimensional culture using spermatogonial stem cells and scaffolds can be used in vitro for induction of spermatogenesis, but there are further technical and ethical challenges in the path of fertile sperm production for the treatment of infertility.  

Porcine spermatogonial stem cells self-renew effectively in a three dimensional culture microenvironment

2017 ◽  
Vol 41 (12) ◽  
pp. 1316-1324 ◽  
Author(s):  
Ji Eun Park ◽  
Min Hee Park ◽  
Min Seong Kim ◽  
Yeo Reum Park ◽  
Jung Im Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
Three Dimensional Culture

scholarly journals Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Vitale Miceli ◽  
Mariangela Pampalone ◽  
Serena Vella ◽  
Anna Paola Carreca ◽  
Giandomenico Amico ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Culture Method ◽  
Paracrine Factors ◽  
Human Amnion ◽  
Tissue Repair And Regeneration ◽  
3D Culture System

The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). The regenerative potential of both 3D culture-derived conditioned medium (3D CM) and their exosomes (EXO) was assessed against 2D culture products. In particular, tubulogenesis assays revealed increased capillary maturation in the presence of 3D CM compared with both 2D CM and 2D EXO. Furthermore, 3D CM had a greater effect on inhibition of PBMC proliferation than both 2D CM and 2D EXO. To support this data, hAMSC spheroids kept in our 3D culture system remained viable and multipotent and secreted considerable amounts of both angiogenic and immunosuppressive factors, which were detected at lower levels in 2D cultures. This work reveals the placenta as an important source of MSCs that can be used for eventual clinical applications as cell-free therapies.

scholarly journals 3D culture technologies of cancer stem cells: promising ex vivo tumor models

2020 ◽  
Vol 11 ◽  
pp. 204173142093340 ◽  
Author(s):  
Chengye Zhang ◽  
Zhaoting Yang ◽  
Da-Long Dong ◽  
Tae-Su Jang ◽  
Jonathan C. Knowles ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Ex Vivo ◽  
Three Dimensional ◽  
3D Culture ◽  
Cell Matrix ◽  
Culture Models ◽  
Three Dimensional Culture ◽  
Three Dimensional Models

Cancer stem cells have been shown to be important in tumorigenesis processes, such as tumor growth, metastasis, and recurrence. As such, many three-dimensional models have been developed to establish an ex vivo microenvironment that cancer stem cells experience under in vivo conditions. Cancer stem cells propagating in three-dimensional culture systems show physiologically related signaling pathway profiles, gene expression, cell–matrix and cell–cell interactions, and drug resistance that reflect at least some of the tumor properties seen in vivo. Herein, we discussed the presently available Cancer stem cell three-dimensional culture models that use biomaterials and engineering tools and the biological implications of these models compared to the conventional ones.

IN VITRO DIFFERENTIATION OF HUMAN SPERMATOGONIAL STEM CELLS IN A THREE DIMENSIONAL (3D) TESTICULAR ORGANOID SYSTEM

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Kara E. McAbee ◽  
Nima Pourhabibi Zarandi ◽  
Anthony Atala ◽  
Hooman Sadri-Ardekani ◽  
Colin Bishop
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation
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