ChemInform Abstract: Nucleosides and Nucleotides. Part 27. Solid Phase Synthesis of a Tridecanucleoside Dodecaphosphate Containing the Unnatural Base 4-Amino-2(1H)-pyridinone and of a Octanucleoside Heptaphosphate for the Determination of the DNA-Polymera

ChemInform ◽  
1987 ◽  
Vol 18 (35) ◽  
Author(s):  
R. CHARCZUK ◽  
C. TAMM
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis

Optimized Automated Solid Phase Synthesis of Oligonucleotides and Derivatives

1998 ◽  
Vol 53 (9) ◽  
pp. 1051-1068 ◽  
Author(s):  
Gabriel Alvarado Urbina ◽  
Gerald Grübler ◽  
Angelika Weiler ◽  
Hartmut Echner ◽  
Stanka Stoeva ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Laser Desorption ◽  
Gene Synthesis ◽  
Chain Reaction ◽  
Laser Desorption Mass Spectrometry ◽  
Phase Synthesis ◽  
Polymerase Chain

An optimized automated synthesizer is presented for assembling oligonucleotides, thiooligonucleotides and 5′-modified oligonucleotides including: chemical phosphorylation, multihydroxyl derivatization with a non-nucleosidic phosphoramidite. The incorporation of biotin, fluorescein and rhodamine phosphoramidites is described. The purification and structure determination of oligo-nucleotides was confirmed using high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and laser desorption mass spectrometry (LDMS). Several applications and confirming data will be presented for gene synthesis and polymerase chain reaction (PCR) experiments.

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