Optimized Automated Solid Phase Synthesis of Oligonucleotides and Derivatives
1998 ◽
Vol 53
(9)
◽
pp. 1051-1068
◽
Keyword(s):
An optimized automated synthesizer is presented for assembling oligonucleotides, thiooligonucleotides and 5′-modified oligonucleotides including: chemical phosphorylation, multihydroxyl derivatization with a non-nucleosidic phosphoramidite. The incorporation of biotin, fluorescein and rhodamine phosphoramidites is described. The purification and structure determination of oligo-nucleotides was confirmed using high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and laser desorption mass spectrometry (LDMS). Several applications and confirming data will be presented for gene synthesis and polymerase chain reaction (PCR) experiments.