ChemInform Abstract: In vitro Prenylation of Aromatic Intermediates in the Biosynthesis of Bitter Acids in Humulus lupulus.

ChemInform ◽  
2010 ◽  
Vol 30 (20) ◽  
pp. no-no
Author(s):  
Karin W. M. Zuurbier ◽  
Suen-Ying Fung ◽  
Johannes J. C. Scheffer ◽  
Robert Verpoorte
Keyword(s):  
Humulus Lupulus ◽  
Bitter Acids

In-vitro prenylation of aromatic intermediates in the biosynthesis of bitter acids in humulus lupulus

1998 ◽  
Vol 49 (8) ◽  
pp. 2315-2322 ◽  
Author(s):  
Karin W.M. Zuurbier ◽  
Suen-Ying Fung ◽  
Johannes J.C. Scheffer ◽  
Robert Verpoorte
Keyword(s):  
Humulus Lupulus ◽  
Bitter Acids

scholarly journals FLAVONOLS HPLC ANALYSIS, IN VITRO BIOLOGICAL ACTIVITIES IN SELECTED HUMULUS LUPULUS L. GENOTYPES

2013 ◽  
Vol 12 (2) ◽  
pp. 129-140
Author(s):  
Maria Maliarova ◽  
Tibor Maliar ◽  
Jana Girmanova ◽  
Jozef Lehotay ◽  
Jan Kraic
Keyword(s):  
Biological Activities ◽  
Humulus Lupulus ◽  
Point Of View ◽  
Raw Material ◽  
Humulus Lupulus L ◽  
Bitter Acids ◽  
High Level ◽  
Beer Production ◽  
Structural Classes

Abstract The Humulus lupulus L. is well known as necessary raw material for beer production. The main structural classes of chemical compounds identified from hop cones include terpenes, bitter acids, prenylated chalcones, and flavonol glycosides. They were subjects of presented work. The content of quercetin was found in the range 490 - 1092 μg/g and that of kaempferol from 218 to 568 μg/g of the dry hop cones. The content of isorhamnetin was very low in all varieties. From biological activities in vitro point of view, relative high level of inhibition activity was observed for six hop genotypes - Zlatan, Lučan, and the Oswald's clones 31, 70, 71, 72, 114 on both enzymes thrombin and urokinase, but without correlation to analyzed flavonols content. In spite of this, antioxidant activity, measured by both the BCLM and HPE methods, was found high and seem to be in correlation with content of analyzed flavonols. Particularly the Oswald's clone 114 expressed very potent biological activities. In general, obtained results indicate that hop cones are valuable material also for other application others than beer production.

scholarly journals Differential regulation of detoxification enzymes in hepatic and mammary tissue by hops (Humulus lupulus ) in vitro and in vivo

2013 ◽  
Vol 57 (6) ◽  
pp. 1055-1066 ◽  
Author(s):  
Birgit M. Dietz ◽  
Ghenet K. Hagos ◽  
Jillian N. Eskra ◽  
Gihani T. Wijewickrama ◽  
Jeffrey R. Anderson ◽  
...  
Keyword(s):  
Differential Regulation ◽  
Humulus Lupulus ◽  
Detoxification Enzymes ◽  
Mammary Tissue

Fast Sampling, Analyses and Chemometrics for Plant Breeding: Bitter Acids, Xanthohumol and Terpenes in Lupulin Glands of Hops (Humulus lupulus)

2016 ◽  
Vol 28 (1) ◽  
pp. 50-57 ◽  
Author(s):  
Daniel P. Killeen ◽  
Oliver C. Watkins ◽  
Catherine E. Sansom ◽  
David H. Andersen ◽  
Keith C. Gordon ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Humulus Lupulus ◽  
Bitter Acids

scholarly journals Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin

2019 ◽  
Vol 72 (2) ◽  
Author(s):  
Michaela Švécarová ◽  
Božena Navrátilová ◽  
Petr Hašler ◽  
Vladan Ondřej
Keyword(s):  
Chromosome Doubling ◽  
Humulus Lupulus ◽  
Nodal Segments ◽  
Root Tips ◽  
Efficient Procedure ◽  
In Vitro Micropropagation ◽  
Humulus Lupulus L ◽  
Artificial Induction ◽  
Internal Standardization

The aim of the research was to establish an efficient procedure for in vitro micropropagation in order to obtain numbers of identical plants for in vitro polyploidization of <em>Humulus lupulus</em> (2<em>n</em> = 20), using antimicrotubule agent oryzalin. For this purpose, the polyploidization was carried out for <em>H. lupulus</em> Osvald’s clones 31, 74, 114, and for ‘Sladek’ cultivar. The two experimental methods – the cultivation of nodal segments on medium with different concentrations of oryzalin (1, 5, and 10 µM) for 2 weeks and the irrigation of nodal segments with oryzalin (10 and 20 µM) for 24 and 48 h were chosen for inducing for polyploid plantlets of <em>H. lupulus</em>. This procedure provided tetraploids, which were identified by flow cytometry using internal standardization method and confirmed using chromosome counting of methaphasic cells from the root tips and morphological observations. The influence of chromosome doubling was also verified with stomata characterization. The polyploid plants were propagated for next evaluation, rooting and transfer to nonsterile conditions and into field. After chromosome doubling, using some different concentration of oryzalin, some plantlets became tetraploids, no mixoploids were detected. The highest efficiency of polyploidization was achieved for clone 72 after 2-week treatment of oryzalin supplemented medium. On the other hand, for method based on the irrigation of nodal segments with oryzalin, the most efficient conditions were treatment with 10 µM and 20 µM oryzalin for 24 and 48 h, respectively.

Hop bitter acids exhibit anti-fibrogenic effects an hepatic stellate cells in vitro

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
M Saugspier ◽  
C Dorn ◽  
M Gehrig ◽  
J Heilmann ◽  
C Hellerbrand
Keyword(s):  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Bitter Acids

scholarly journals Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 159-164
Author(s):  
Josef Patzak ◽  
Alena Henychová ◽  
Petr Svoboda ◽  
Ivana Malířová
Keyword(s):  
De Novo ◽  
Molecular Genetic ◽  
Methylation Status ◽  
Hierarchical Cluster ◽  
Humulus Lupulus ◽  
Field Conditions ◽  
Meristem Culture ◽  
The Individual ◽  
De Novo Methylation

In vitro meristem cultures have been used for the production of hop (Humulus lupulus L.) virus-free rootstocks worldwide, because multipropagation is considered to preserve the genetic stability of the produced plantlet. Nevertheless, in vitro tissue cultures can cause genetic and epigenetic changes. Therefore, we studied the genetic and epigenetic variability of Saaz Osvald’s clones, Sládek and Premiant cultivars on the DNA methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro propagated plants, acclimatised glasshouse rootstocks as well as derived mericlones and control plants under field conditions were used for the analyses. A total of 346 clearly and highly reproducible amplified products were detected in the MSAP analyses within the studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. The demethylation events were comparable to the de novo methylation events. Most demethylation changes were found in the in vitro plants, but only a few of them were found in the derived mericlones under field conditions. In contrast, the de novo methylation changes persisted in the acclimatised plants under glasshouse or field conditions. A hierarchical cluster analysis was used for the evaluation of the molecular genetic variability within the individual samples. The dendrogram showed that the individual samples of the same variety, more or less, clustered together. Because the methylation status varied during the virus-free rootstock production process, we suppose that de/methylation process is a natural tool of epigenetics and evolution in vegetatively propagated plants.

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