Normal phase chiral HPLC of methylphenidate: Comparison of different polysaccharide-based chiral stationary phases

Chirality ◽  
2001 ◽  
Vol 14 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Hassan Y. Aboul-Enein ◽  
Imran Ali
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Normal Phase ◽  
Chiral Hplc

scholarly journals The Separation of Cannabinoids on Sub-2 µm Immobilized Polysaccharide Chiral Stationary Phases

2021 ◽  
Vol 14 (12) ◽  
pp. 1250
Author(s):  
Takafumi Onishi ◽  
Weston J. Umstead
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Elution Order ◽  
Testing Methods ◽  
Structural Isomers

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

Enantiomeric separation of new phytoalexin analogs with cyclofructan chiral stationary phases in normal-phase mode

2016 ◽  
Vol 39 (19) ◽  
pp. 3669-3676 ◽  
Author(s):  
Marianna Moskaľová ◽  
Ján Petrovaj ◽  
Taťána Gondová ◽  
Mariana Budovská ◽  
Daniel W. Armstrong
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Enantiomeric Separation ◽  
Normal Phase

Analysis of Orphenadrine Citrate in Various Chiral Stationary Phases: A Comparative Study

2020 ◽  
Vol 7 (2) ◽  
pp. 91-100
Author(s):  
Prachi Raikar ◽  
Bannimath M. Gurupadayya ◽  
Sripuram Subramanyam ◽  
Gunnam Srinivasu
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Enantiomeric Separation ◽  
Normal Phase ◽  
Good Resolution ◽  
Reverse Phase ◽  
Polar Mode ◽  
Ich Guidelines ◽  
Chiralcel Od ◽  
Fast Liquid Chromatography

Background: Polysaccharide based chiral stationary phases (CSPs) were used to perform enantiomeric separation of Orphenadrine Citrate by Ultra-Fast Liquid Chromatography (UFLC) technique. Trials were conducted using the polar mode, reverse phase mode and normal phase mode. Amylose and Cellulose-based CSPs were used for the same. Materials and Methods: Eight Amylose-based CSPs and four Cellulose-based CSPs were used in the reverse phase mode. Five Amylose-based CSPs and two Cellulose-based CSPs were used in polar mode. The only Cellulose-based CSP used in the normal phase mode could effectively separate Orphenadrine Citrate enantiomers with a good resolution. Results and Discussion: Successful enantioseparation was obtained using Chiralcel OD-H containing Cellulose tris (3, 5- dimethylphenylcarbamate) as a chiral selector and n-hexane: Ethanol: Diethylamine (95: 05: 0.1, v/v/v) as the mobile phase. The developed method was validated in accordance with ICH guidelines (Q2R1). Conclusion: The proposed objectives were successfully accomplished as the developed method could effectively resolve Orphenadrine Citrate enantiomers.

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