Development of dissolved carbon dioxide‐driven‐and‐controlled repeated batch fermentation process for ethanol production

2020 ◽  
Vol 98 (12) ◽  
pp. 2507-2515
Author(s):  
Sijing Feng ◽  
Yen‐Han Lin
AMB Express ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Nikolay Vassilev ◽  
Bettina Eichler-Löbermann ◽  
Elena Flor-Peregrin ◽  
Vanessa Martos ◽  
Antonia Reyes ◽  
...  

1998 ◽  
Vol 64 (7) ◽  
pp. 2644-2651 ◽  
Author(s):  
Gijs Gerritse ◽  
Ronald W. J. Hommes ◽  
Wim J. Quax

ABSTRACT Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on the secretion of the alkaline lipase from P. alcaligenes was developed. Due to the inability of P. alcaligenes to grow on glucose, citric acid and soybean oil were applied as substrates in the batch phase and feed phase, respectively. The gene encoding the high-alkaline lipase from P. alcaligenes was isolated and characterized. Amplification of lipase gene copies in P. alcaligenes with the aid of low- and high-copy-number plasmids resulted in an increase of lipase expression that was apparently colinear with the gene copy number. It was found that overexpression of the lipase helper gene,lipB, produced a stimulating effect in strains with high copy numbers (>20) of the lipase structural gene, lipA. In strains with lipA on a low-copy-number vector, thelipB gene did not show any effect, suggesting that LipB is required in a low ratio to LipA only. During scaling up of the fermentation process to 100 m3, severe losses in lipase productivity were observed. Simulations have identified an increased level of dissolved carbon dioxide as the most probable cause for the scale-up losses. A large-scale fermentation protocol with a reduced dissolved carbon dioxide concentration resulted in a substantial elimination of the scale-up loss.


2011 ◽  
Vol 111 (4) ◽  
pp. 433-436 ◽  
Author(s):  
Shigeru Chohnan ◽  
Megumi Nakane ◽  
M. Habibur Rahman ◽  
Youji Nitta ◽  
Takanori Yoshiura ◽  
...  

2013 ◽  
Vol 196 (2) ◽  
pp. 97-107 ◽  
Author(s):  
Stefano Romano ◽  
Patrizia Paganin ◽  
Cristiano Varrone ◽  
Silvia Tabacchioni ◽  
Luigi Chiarini

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